tissue culture
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"tissue culture." A Dictionary of Plant Sciences. . Encyclopedia.com. 22 Feb. 2019 <https://www.encyclopedia.com>.
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Tissue Culture
Tissue Culture
For a variety of purposes, plant cells, tissues, organs, and whole plants can be grown in labware containing a medium composed of defined molecules. Tissue culture media provide water, minerals, vitamins, hormones, carbon sources, and antibiotics depending on the plant material being cultured. Since most living plant cells are totipotent , scientists can manipulate the medium to regenerate whole plants from even a single genetically engineered plant cell or from a cluster of cells from a rare plant. Hormones in the medium determine what plant parts form from the cells (callus) in the culture: Auxins stimulate root formation and cytokinins stimulate shoot formation. The medium may contain agar, agarose, phytagel, or other polysaccharides to form a semisolid gel to support the plant tissue. The container must be colorless if photosynthesis is to be supported, and the light source should not be too intense to avoid the greenhouse effect inside the container. If the container is not ventilated, a carbon source such as sucrose will have to be used. Tissues must be subcultured periodically to avoid solute concentration buildup if the container is ventilated. Antibiotics may be used to keep the culture clear of bacteria, fungi, or other contaminants or to select for genetically engineered cells. Tissue culture techniques are also used to generate large numbers of genetically identical plants for agricultural applications, or to generate additional plants of rare or endangered species.
see also Genetic Engineering; Propagation; Reproduction, Asexual; Transgenic Plants.
Ross Koning
Bibliography
Hopkins, W. G. Introduction to Plant Physiology, 2nd ed. New York: John Wiley & Sons, 1999.
Raven, Peter. H., Ray F. Evert, and Susan E. Eichhorn. Biology of Plants, 6th ed. New York: W. H. Freeman and Co., 1999.
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tissue culture
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"tissue culture." A Dictionary of Biology. . Encyclopedia.com. 22 Feb. 2019 <https://www.encyclopedia.com>.
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tissue culture
tissue culture, the propagation of plants through the placement of small amounts of undifferentiated tissue or single cells in an artificial environment. The tissue is placed in a nutrient medium that favors the production of roots and shoots, and is later planted normally. By using tissue culture, the favorable qualities of plants can be precisely controlled, so that each plant is identical for the particular quality being sought, whether it be disease resistance or plant chemical production. In 2012 the technique was reported to have been used by Russian scientists to grow plants from Siberian campion fruits dated at 31,800 years old.
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tissue culture
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"tissue culture." A Dictionary of Ecology. . Encyclopedia.com. 22 Feb. 2019 <https://www.encyclopedia.com>.
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"tissue culture." A Dictionary of Ecology. . Retrieved February 22, 2019 from Encyclopedia.com: https://www.encyclopedia.com/science/dictionaries-thesauruses-pictures-and-press-releases/tissue-culture
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