Recombinant deoxyribonucleic acid (DNA) technology allows the creation and manipulation of DNA sequences that come from different sources, even different species. The development of recombinant DNA technology in the 1970s was hailed as the most exciting invention since the development of transistors some twenty to thirty years earlier. The transistor changed people's lives forever by creating the microelectronics revolution and enabling the development of portable radios, tape and compact disc players, cellular phones, and computers, all leading to fabulous wealth in the developed world. Recombinant DNA technology is likely to also have profound effects on society, including better health through improved disease diagnosis, much better understanding of human gene variation, improved drug and pharmaceutical production, vastly more sensitive and specific crime scene forensics , and production of genetically modified organisms that significantly improve yields and nutritional value of crops while decreasing reliance on pesticides and artificial fertilizers. Recombinant DNA and the transgenic technology that it spawned have already entered everyday lives to a degree, as evidenced by the completion of a draft of the human genome sequence, criminal trials relying on DNA evidence, and controversy over the use of genetically modified corn and other organisms.
Recombinant DNA technology has had to create its place instead of entering an existing market. As a result, recombinant DNA technology has probably consumed more finances than it has yet generated, although this discounts the long-term value of increasing knowledge. Where recombinant DNA technology has made the biggest economic impact is in the pharmaceutical industry, allowing the production of single human proteins for therapeutic use or to generate specific antibodies. Harvesting human insulin created in bacterial cells is far easier than isolating it from pig or human cadaver pituitary glands, for instance. The financial base for recombinant DNA technology should continue to improve as genetically modified organisms are becoming widely used in agriculture; more than half the U.S. soybean crop now consists of a strain genetically modified to reduce the amount of herbicides necessary to bring in a good yield.
A clone is a collection of organisms that are genetically identical, and a recombinant DNA clone is a collection of genetically identical organisms (most often bacteria) that each carry a specific foreign (from another source) DNA molecule. "Clone" also refers to the foreign DNA itself after being placed in the target organism. Thus, scientists would speak of the "cloned DNA." Typically, a specific DNA molecule is inserted into a vector DNA molecule that can carry foreign DNA, and the resulting recombinant DNA is introduced into a host organism (often the common bacterium Escherichia coli or the yeast Saccharomyces cerevisiae ). Large numbers of genetically identical host organisms, each carrying the same specific foreign DNA molecule, can be produced, allowing the DNA or its protein product to be produced in large quantities.
The process of DNA cloning has two components. One is the use of restriction enzymes in vitro to cut DNA into a unique set of fragments. Restriction enzymes are endonucleases that bacteria naturally use to defend against DNA viruses by cleaving DNA at specific sites. The enzyme EcoRI, for example, from E. coli, cleaves every site with the six-nucleotide sequence of GAATTC, found on average every 4,100 nucleotides in DNA. (A companion methylase enzyme modifies the bacterium's own GAATTC sites so they are not targets of EcoRI.) Researchers have isolated many different restriction enzymes from bacterial species. The enzymes differ in the sequences of the target sites that they cut, in the locations of the cleavage sites, and by whether modified target sites are cleaved (in some cases modification is required for cleavage). The collection of restriction enzymes with these different properties provides an invaluable toolbox for cutting and joining DNA molecules from different sources.
The other component of DNA cloning technology is the use of vectors to ensure that the host organism carries and replicates the foreign DNA. Most often bacteria are used as the host organism, because of their fast growth and the ready availability of techniques for manipulating and growing bacteria in small- and large-scale cultures. Vectors are DNA molecules that contain an origin of replication that functions in the host organism (to allow the vector to be copied), and a gene that confers some survival advantage on host cells that contain the vector DNA. Typically the vector carries a gene that confers resistance to a particular drug, such as an antibiotic.
The original vectors used were based on naturally occurring small, circular DNA molecules distinct from the bacterial chromosome , called plasmids . The most widely used vector of the late 1970s to early 1980s was the plasmid pBR322, which contained an origin of replication, a gene that confers resistance to the antibiotic ampicillin, and a second gene that confers resistance to the antibiotic tetracycline. Each of these antibiotic resistance genes contains the recognition sequence for a restriction endonuclease. Opening the vector at one of those sites by restriction digestion in vitro and ligating (splicing) the foreign DNA into that site destroys the resistance encoded by that gene but leaves the other resistance factor intact. Plasmid DNA can then be put back into bacterial host cells (by transfection) where it can replicate up to several hundred copies per bacterium. The bacteria are then grown in media containing one or the other antibiotic. This facilitates selection and identification of bacteria receiving the ligation product. The cloned DNA then replicates along with the rest of the plasmid DNA to which it is joined.
Later improved editions of plasmid vectors incorporated such features as polylinker sequences that consist of several unique restriction sites close together to form a specific cloning site and inducible promoter sequences adjacent to the polylinker used to transcribe into RNA, or "express," the cloned DNA as desired.
cDNAs and Gene Analysis
Plasmid vectors are limited in the size of the cloned DNA that can be incorporated and successfully reintroduced into the bacterium, typically holding a maximum of about 15 kb (kilobases [1 kb equals 1,000 bases]) of foreign DNA. One common use for plasmid vectors is to make cDNA (complementary DNA) libraries; cDNA molecules are DNA copies of messenger ribonucleic acid (mRNA) molecules, produced in vitro by action of the enzyme reverse transcriptase . Because cDNAs represent only the portions of eukaryotic genes that are transcribed into the mRNA, cDNA clones are particularly useful for analysis of gene expression and cell specialization. The existence of a cDNA is also evidence that the gene is active, or transcribed, in the cells or tissues from which the mRNA was isolated. Such information can be used to compare gene activities in healthy versus diseased cells, for instance.
Frequently the simpler sequence of a cDNA is easier to analyze than the corresponding genomic sequence since it will not contain noncoding, or intervening, sequences (introns). Another advantage of cDNA is that generally the sequence does not include enhancers or regulatory sequences to direct their transcription. As a result, they can be combined with other regulatory systems in the clone to direct their expression.
Genome sequencing projects typically generate sequence information from many different cDNA clones. The cDNA cloned sequence is termed an "expressed sequence tag" (EST), and, when correlated with the whole genomic DNA sequence, EST information can help determine the locations and sizes of genes.
In order to obtain the cDNA for a specific gene, it is first necessary to construct a cDNA "library." This is a collection of bacteria that contain all the cDNAs from the cell or tissue type of interest. To make a library, the thousands of different mRNAs are first harvested from the cell of interest, and cDNA is made using reverse transcriptase. The cDNA is then cloned into plasmids, and introduced into bacteria. Under the right conditions, each bacterium will take up only one cDNA. The bacteria are then grown in Petri dishes on a solid medium. A library therefore consists of a mixed population of bacteria, each carrying one type of cDNA. To find the bacterium containing a particular type of cDNA, one can either search for the gene itself with a nucleotide probe or for its protein product with an antibody .
Screening a library depends either on having a probe bearing part of the nucleotide sequence or an antibody or other way of recognizing the protein coded by the gene. Screening by nucleotide probes (labeled with radioactive or chemical tags for detection) depends on base pair complementarity between the single-stranded target DNA and the probe DNA; this allows the label to mark the cell with the desired cDNA. Screening by labeled antibody depends on binding of the antibody to the protein encoded by the gene. Literally thousands of cloned genes have been isolated this way from libraries of many different species. One of the most powerful observations in biology is that the same or similar gene sequences can be isolated from different species, ranging from bacteria to humans.
Human insulin was the first medicine to be created through recombinant DNA technology. Insulin is a protein hormone produced by the pancreas that is vital for regulation of blood sugar. In the disease insulin-dependent diabetes mellitus (IDDM), the immune system attacks and destroys the insulin-producing cells. A person with IDDM requires daily injections of insulin to control blood sugar. Before 1980, insulin was isolated from pigs or other animals. Animal insulin has a slightly different amino acid sequence from the human form. In the early 1980s, recombinant DNA technology was used to splice the human insulin gene into bacteria, which were grown in vats to make large amounts of the human protein. Recombinant human insulin was the first recombinant drug approved for human use. Since then more than two dozen other drugs have been created in this way, including growth hormone, blood clotting factors, and tissue plasminogen activator, used to break up blood clots following a stroke. Gene sequence similarities indicate that all living organisms have descended from shared common ancestors, back to the beginning of life.
Cloned DNA can also be incorporated into the genomes of multicellular organisms to create a transgenic organism. This makes possible a new approach to designing genotypes by adding genes (gene-coded functions) to species where those genes (functions) do not exist. Genetically modified organisms (GMOs) created by modifying a gene or adding one from another species frequently offer the most direct way to improve the way people use organisms for food or chemistry.
One example of a GMO is the development of "golden rice," designed to reduce blindness caused by vitamin A deficiency in rice-consuming areas of the world. A polished rice grain, which is the portion of the seed that provides nourishment (the endosperm ) does not contain beta-carotene, the substance the human body converts into vitamin A, yet many plants with yellow/orange colored leaves or flowers produce it in abundance. To convert rice endosperm into a beta-carotene-rich food, a transgene was constructed with the genes required for beta-carotene production and inserted into rice cells. The transgene consists of a cDNA for phytoene synthase, from a daffodil flower library, plus other sequences. Rice with these extra genes show a rich "golden" color from the beta-carotene that accumulates in the rice grain. If golden rice can be bred into commercial strains and enough can be provided into the diet to reduce the incidence of vitamin A–related blindness, current agitation against GMO crops may evolve into enthusiasm for their application.
Genome Libraries: Sequencing Genomes
Recall that cDNAs do not contain introns . Comparing a cDNA sequence with its corresponding DNA sequence on a chromosome (the genomic sequence) reveals the locations of introns in the genomic sequences. Genomic DNA libraries, in which the cloned DNA originates from fragments of the chromosomal DNA, carry intronic sequences, as well as the DNA between genes. In the more complex eukaryotes the same genomic region may correspond to several different cDNAs. This reveals the existence of alternative splicing, in which different sets of exons are used to make separate mRNA transcripts from one gene region. This expands the diversity of the protein, encoded by a single gene to include slightly different protein forms, called isoforms. Tissue-specific regulation of splicing indicates that these isoforms contribute important nuances to creating developmental differences between tissues.
Genomic DNA libraries have also proved invaluable for isolating genes that are poorly expressed (that is, make little mRNA) and for mapping disease-causing genes to specific chromosomal sites. The vectors used in genomic libraries are designed to incorporate greater lengths of cloned DNA than plasmids can carry. The first of these vectors was the lambda bacterial virus, which could hold an insert of 15 kb, followed by the cosmid, a hybrid between a plasmid and a phage (a virus that infects bacteria) with a DNA insert size of 45 kb. Development of linear yeast artificial chromosomes (YACs), which include a yeast centromere , origin of replication, and ends (telomeres), which successfully grow in the yeast Saccharomyces cerevisiae, carry clones of 200 kb to more than 2,000 kb. Subsequent development of bacterial artificial chromosomes (BACs) that contain 100 kb of insert DNA and are relatively easy to culture has put genomic cloning within reach of almost every molecular biology laboratory. (Clones are harder to work with as they get larger.)
BACs provided one route to sequencing the human genome, where their large capacity was critical. All the different genome sequencing projects start with a large number of BAC clones for that species, subclone 1 kb fragments of the DNA from each BAC into plasmids, and determine their sequence using high-speed machines. Computer-based comparisons of the results then assemble the nucleotide sequences into a coherent order by aligning the regions where they overlap.
A library of genomic DNA contains many clones with inserts that partially overlap each other because random breakage of chromosomal DNA is used to produce fragments for cloning. The order of fragments in the original chromosome can be determined by "chromosome walking." In this technique, a portion (subclone) from one clone is used as a probe to identify another clone that also carries that sequence. The two clones are then compared, and the nonoverlapping end of the second clone is subcloned for use as the next probe. In this way, a "walk" is carried out over many steps to identify adjacent DNA on the same chromosome, allowing the fragments to be placed in sequence. A series of sequential, partially overlapping clones is termed a "contig" (for contiguous sequence); the goal of genome mapping is to make a separate contig for all the DNA clones from one chromosome (a continuous covalent molecule). Contigs made large genome sequencing feasible since a minimum number of BACs could be chosen from their order in the map.
Finding Disease Genes
Locating a human disease gene on a chromosome map is now equivalent to locating the gene (approximately) on a contig and the DNA sequence map. This speeds gene identification through cloning the gene and determining what protein the gene encodes. The positional approach is important for single-gene (Mendelian) disease traits that are well known clinically but not at a biochemical level.
Cystic fibrosis (CF) was one such disease. It is the most common severe autosomal recessive disorder among European populations and their descendants in the New World. Patients suffer from mucus accumulation and frequent bacterial infections in their lungs. In the United States, CF patients are the single largest group receiving transplants to replace damaged lungs. However, the clinical studies failed to determine which gene product is defective in the patients. Extensive studies on families with CF led to identification of the causative gene on chromosome 7.
Initially recombination studies placed the gene within a small region of the chromosome of approximately one million base pairs. Starting at DNA clones from both ends of this region, the researchers used chromosome walking to clone all of the interval; several candidate genes were identified within the region but rejected as the cause of CF. Finally, one gene was identified within these clones that had the right properties: It was normally expressed in the lungs but not the brain, and it encoded a protein that made sense for the cause of the disease. In addition, patients with CF had specific mutations in this gene. The functional CF gene encodes a chloride channel transmembrane regulatory protein (CFTR) that controls transport of certain ions in and out of epithelial (surface) cells. The most common mutation encodes a CFTR protein that is missing one amino acid and cannot reach its site of function in the cell membrane. As a result, ions become too concentrated inside the cell, and water moves in. The result is dried secretions , such as very sticky mucus.
Gene therapy to add a functional copy of the CFTR to lung cells has not been successful, in part because the patients develop an immune response to reject the vector, and, in some cases, the normal protein. Mild improvements have been short-lived, or affect only small patches of cells in the respiratory tract. Alternative approaches to better understanding the physiology of the disease to direct drug design seem more viable. To this end, a mouse model with an inactivated CFTR gene is used to test potential drugs.
see also Bacterial Genetics; Bioinformatics; Clone; DNA Sequencing; Forensic DNA Analysis; Gene Therapy; Genetic Diseases; Genome; Genomics; Human Genome Project; Radiation Hybrid Mapping; Reverse Transcriptase; Transgenic Techniques
Alberts, Bruce, et al. Molecular Biology of the Cell, 4th ed. New York: Garland Publishing, 2000.
Felsenfeld, Gary. "DNA." Scientific American 253 (1985): 58–67.
Watson, James D., and Francis H. Crick. "A Structure for Deoxyribose Nucleic Acid." Nature 171 (1953): 737.
Watson, James D., Michael Gilman, Jan Witkowski, and Mark Zoller. Recombinant DNA, 2nd ed. New York: Scientific American Books, 1992.
Recombinant DNA refers to a collection of techniques for creating (and analyzing) DNA molecules that contain DNA from two unrelated organisms. One of the DNA molecules is typically a bacterial or viral DNA that is capable of accepting another DNA molecule; this is called a vector DNA. The other DNA molecule is from an organism of interest, which could be anything from a bacterium to a whale, or a human. Combining these two DNA molecules allows for the replication of many copies of a specific DNA. These copies of DNA can be studied in detail, used to produce valuable proteins, or used for gene therapy or other applications.
The development of recombinant DNA tools and techniques in the early 1970s led to much concern about developing genetically modified organisms with unanticipated and potentially dangerous properties. This concern led to a proposal for a voluntary moratorium on recombinant DNA research in 1974, and to a meeting in 1975 at the Asilomar Conference Center in California. Participants at the Asilomar Conference agreed to a set of safety standards for recombinant DNA work, including the use of disabled bacteria that were unable to survive outside the laboratory. This conference helped satisfy the public about the safety of recombinant DNA research, and led to a rapid expansion of the use of these powerful new technologies.
Overview of Recombination Techniques
The basic technique of recombinant DNA involves digesting a vector DNA with a restriction enzyme , which is a molecular scissors that cuts DNA at specific sites. A DNA molecule from the organism of interest is also digested, in a separate tube, with the same restriction enzyme. The two DNAs are then mixed together and joined, this time using an enzyme called DNA ligase, to make an intact, double-stranded DNA molecule. This construct is then put into Escherichia coli cells, where the resulting DNA is copied billions of times. This novel DNA molecule is then isolated from the E. coli cells and analyzed to make sure that the correct construct was produced. This DNA can then be sequenced, used to generate protein from E. coli or another host, or for many other purposes.
There are many variations on this basic method of producing recombinant DNA molecules. For example, sometimes researchers are interested in isolating a whole collection of DNAs from an organism. In this case, they digest the whole genome with restriction enzyme, join many DNA fragments into many different vector molecules, and then transform those molecules into E. coli. The different E. coli cells that contain different DNA molecules are then pooled, resulting in a "library" of E. coli cells that contain, collectively, all of the genes present in the original organism.
Another variation is to make a library of all expressed genes (genes that are used to make proteins) from an organism or tissue. In this case, RNA is isolated. The isolated RNA is converted to DNA using the enzyme called reverse transcriptase. The resulting DNA copy, commonly abbreviated as cDNA, is then joined to vector molecules and put into E. coli. This collection of recombinant cDNAs (a cDNA library) allows researchers to study the expressed genes in an organism, independent from nonexpressed DNA.
Recombinant DNA technology has been used for many purposes. The Human Genome Project has relied on recombinant DNA technology to generate libraries of genomic DNA molecules. Proteins for the treatment or diagnosis of disease have been produced using recombinant DNA techniques. In recent years, a number of crops have been modified using these methods as well.
As of 2001, over eighty products that are currently used for treatment of disease or for vaccination had been produced using recombinant DNA techniques. The first was human insulin, which was produced in 1978. Other protein therapies that have been produced using recombinant DNA technology include hepatitis B vaccine, human growth hormone, clotting factors for treating hemophilia, and many other drugs. At least 350 additional recombinant-based drugs are currently being tested for safety and efficacy. In addition, a number of diagnostic tests for diseases, including tests for hepatitis and AIDS, have been produced with recombinant DNA technology.
Gene therapy is another area of applied genetics that requires recombinant DNA techniques. In this case, the recombinant DNA molecules themselves are used for therapy. Gene therapy is being developed or attempted for a number of inherited human diseases.
Recombinant DNA technology has also been used to produce genetically modified foods. These include tomatoes that can be vine-ripened before shipping and rice with improved nutritional qualities. Genetically modified foods have generated controversy, and there is an ongoing debate in some communities about the benefits and risks of developing crops using recombinant DNA technology.
Since the mid-1970s, recombinant DNA techniques have been widely applied in research laboratories and in pharmaceutical and agricultural companies. It is likely that this relatively new area of genetics will continue to play an increasingly important part in biological research into the foreseeable future.
see also Biotechnology; Cloning Genes; Crossing Over; DNA Libraries; Escherichia Coli; Gene Therapy: Ethical Issues; Genetically Modified Foods; Human Genome Project; Plasmid; Restriction Enzymes; Reverse Transcriptase; Transposable Genetic Elements.
Patrick G. Guilfoile
Cooper, Geoffrey. The Cell: A Molecular Approach. Washington, DC: ASM Press, 1997.
Glick, Bernard, and Jack Pasternak. Molecular Biotechnology: Principles and Applications of Recombinant DNA, 2nd ed. Washington, DC: ASM Press, 1998.
Kreuzer, Helen, and Adrianne Massey. Recombinant DNA and Biotechnology, 2nd ed. Washington, DC: ASM Press, 2000.
Lodish, Harvey, et al. Molecular Cell Biology, 4th ed. New York: W. H. Freeman, 2000.
Old, R. W., and S. B. Primrose. Principles of Gene Manipulation, 5th ed. London: Blackwell Scientific Publications, 1994.
"Approved Biotechnology Drugs." Biotechnology Industry Organization. <http://www.bio.org/aboutbio/guide2.html>.
Recombinant DNA (rDNA) is made of segments of DNA (polymers of deoxyribonucleotides) from two or more sources. Nature has been recombining DNA in living cells for eons, but humans have only recently discovered the means to carry out this operation in the test tube. DNA is the molecule of heredity, and the procedure used for preparing rDNA is referred to as genetic engineering. The biotechnology industry and much of modern medicine, basic research, and agriculture depend on the use of rDNA.
How the Technology Developed
The discovery of restriction endonucleases, enzymes that reproducibly cleave double-stranded DNA molecules at specific sequences, paved the way for the development of rDNA technology. Restriction endonucleases are produced in bacteria as a defense mechanism of that bacterium to restrict the growth of invading bacterial viruses; they act by destroying viral DNA. The enzymes hydrolyze or "cut" specific sites within a DNA molecule. In 1978, the Nobel Prize in medicine was awarded to Werner Arber, Daniel Nathans, and Hamilton O. Smith for the discovery and investigation of restriction endonucleases. The cuts made by these enzymes often leave single DNA strands with sticky ends due to the asymmetry of the cut (made to a double-stranded molecule) and the tendency of the bases in DNA to form hydrogen bonds with complementary bases on another strand. Scientists realized that these enzymes could serve as a powerful tool for manipulating DNA in a controlled way. The 1980 Nobel Prize in chemistry was awarded to Paul Berg for constructing the first recombinant DNA molecules.
When scientists became concerned about whether this new technology posed risks to humans and the environment, there was an unprecedented (and temporary) suspension of experiments using rDNA. In 1975, a conference was held in Asilomar, California, to assess such risks, and it was determined that most rDNA work should continue as long as appropriate safeguards were in place. A Recombinant DNA Advisory Committee was established through the auspices of the National Institutes of Health to set up guidelines and assess risks and benefits of proposed projects using rDNA. That committee, composed of scientists, physicians, ethicists, and legal experts, has met regularly since that time.
When a DNA carrier, called a vector, and a targeted DNA sample are treated with the same restriction enzyme, the resulting fragments are left with matching, or complementary, sticky ends. When mixed, the two samples of treated DNA will "stick" together, and another enzyme, DNA ligase, will seal pieces of DNA together with the formation of covalent bonds (see Figure 1). Vectors are often plasmids, small extrachromosomal and circular DNA that are incorporated into bacterial DNA such as that of E. coli, so that the inserted or cloned genes can be reproduced manyfold and products of the inserted genes (protein molecules) can be manufactured, sometimes in large quantities. Some vectors are designed to induce protein synthesis from the information in the inserted genes. Other vectors are designed to deliver large segments of DNA to specific cells.
Uses of rDNA Technology
Essentially every area of biological research has been affected by the use of rDNA technology. Protein structure/function relationship studies and gene expression and regulation research have been enormously enhanced by this powerful tool. Transgenic animals (into which DNA from another species has been inserted) have been bred to expand the study of human biochemical processes and diseases. Transgenic mice that are highly susceptible to breast cancer or Alzheimer's disease have furthered the understanding of those diseases.
Modern medicine is inextricably linked with rDNA technology. Gene therapy replaces defective genes with functional ones, delivered to the patient by way of a suitable vector, usually a disabled virus. The first moderately successful gene therapy was instituted to treat an inborn immune deficiency disease (ADA deficiency) caused by a defective enzyme, adenine deaminase. Cancer research and treatments as well as some vaccine development make use of rDNA technology. Attempts to modify animals genetically in such a way that organs suitable for transplant into humans may be harvested are now being made.
Agricultural uses of recombinant DNA technology are expanding. Genetically engineered bacteria sprayed onto strawberries protect the strawberries from freezing. Genes that promote herbicide resistance are incorporated into plants so that herbicides can be used for no-till farming. Some plant species have been transformed by rDNA containing genes that promote resistance to insects and pathogens.
The industrial use of rDNA technology includes the production of bleach-resistant enzymes that are used in laundry detergents to degrade
proteins. Transgenic cows that produce human milk for use in baby food have been bred. In 1980 the verdict of a landmark case heard before the U.S. Supreme Court stated that a genetically engineered bacterium, designed to digest oil in oil spills by researchers at Exxon, could be patented.
Recombinant DNA technology was used as an artist's tool in a macabre incident in which a jellyfish gene for a green fluorescent protein, often used in research as a marker for gene transfer, was inserted into a rabbit, making the rabbit fluoresce under green light. This and other dubious uses of rDNA technology have engendered a number of ethical, economic, safety, and legal debates.
see also Clones; DNA Replication; Hydrogen; Restriction Enzymes.
Sharron W. Smith
Eun, Hyone-Myong (1996). Enzymology Primer for Recombinant DNA Technology. San Diego: Academic Press.
Garrett, Reginald H., and Grisham, Charles M. (2002). Principles of Biochemistry: With a Human Focus. Fort Worth, TX: Harcourt College Publishers.
National Institutes of Health. Office of Biotechnology Activities. Information available from <http://www4.od.nih.gov/oba/>.
Deoxyribonucleic acid (DNA) is the information blueprint that exists in most living organisms. Some viruses instead contain ribonucleic acid (RNA) . Even these viruses require the production of DNA at some stage of their replication.
DNA from different organisms of the same species combines together naturally to yield an organism that has traits from both parent organisms. There is also evidence accumulating that DNA transfer between different species may be a natural process. However, much interspecies mixing of DNA is the result of deliberate experimental manipulations.
A crucial process of these manipulations is the preparation of recombinant DNA. Recombinant DNA is DNA from different organisms that have been chemically bonded together to form a single DNA. The recombinant DNA can be interpreted by the various enzymes of prokaryotic or eukaryotic cells, so that the genes contained in the recombinant DNA can be expressed and the protein products produced.
The recombination can involve the DNA from two eukaryotic organisms, two prokaryotic organisms, or between an eukaryote and a prokaryote . An example of the latter is the production of human insulin by the bacterium Escherichia coli, which has been achieved by splicing the gene for insulin into the E. coligenome such that the insulin gene is expressed and the protein product formed.
The splicing of DNA from one genome to another is done using two classes of enzymes. Isolation of the target DNA sequence is done using restriction enzymes. There are well over a hundred restriction enzymes, each cutting in a very precise way a specific base of the DNA molecule . Used singly or in combination, the enzymes allow target segments of DNA to be isolated. Insertion of the isolated DNA into the recipient genome is done using an enzyme called DNA ligase.
Typically, the recombinant DNA forms part of the DNA making up a plasmid. A plasmid is a circular piece of DNA that exists outside of the main body of genetic material. The mobility of the plasmid facilitates the easy transfer of the recombinant DNA from the host organism to the recipient organism.
Molecular biologist Paul Berg of Stanford University first achieved the manufacture of recombinant DNA in 1972. Berg isolated a gene from a human cancer-causing monkey virus , and then joined the oncogene into the genome of the bacterial virus lambda. For this and subsequent recombinant DNA studies (which followed a voluntary one-year moratorium from his research while safety issues were addressed) he was awarded the 1980 Nobel Prize in chemistry .
In 1973, Stanley Cohen and Herbert Boyer created the first recombinant DNA organism, by adding recombinant plasmids to E. coli. Since that time, advances in molecular biology techniques, in particular the development of the polymerase chain reaction, have made the construction of recombinant DNA swifter and easier. Cohen and Boyer's accomplishment was the birth of modern biotechnology , and spawned the resulting biotechnology industry.
Recombinant DNA has been of fundamental importance in furthering the understanding of genetic regulatory processes and shows great potential in the genetic design of therapeutic strategies.
Recombinant deoxyribonucleic acid (DNA) is genetic material from different organisms that has been chemically bonded together to form a single macromolecule. The recombination can involve the DNA from two eukaryotic organisms, two prokaryotic organisms, or between an eukaryote and a prokaryote. An example of the latter is the production of human insulin by the bacterium Escherichia coli, which has been achieved by splicing the gene for insulin into the E. coli genome such that the insulin gene is expressed and the protein product formed.
The splicing of DNA from one genome to another is done using two classes of enzymes. Isolation of the target DNA sequence is done using restriction enzymes. There are well over a hundred restriction enzymes, each cutting in a very precise way a specific base of the DNA molecule. Used singly or in combination, the enzymes allow target segments of DNA to be isolated. Insertion of the isolated DNA into the recipient genome is done using an enzyme called DNA ligase.
Typically, the recombinant DNA forms part of the DNA making up a plasmid. The mobility of the plasmid facilitates the easy transfer of the recombinant DNA from the host organism to the recipient organism.
Paul Berg (1926–) of Stanford University first achieved the manufacture of recombinant DNA in 1972. Berg isolated a gene from a human cancer-causing monkey virus, and then ligated the oncogene into the genome of the bacterial virus lambda. For this and subsequent recombinant DNA studies (which followed a voluntary one-year moratorium from his research while safety issues were addressed) he was awarded the 1980 Nobel Prize in chemistry.
In 1973, Stanley Cohen (1922–) and Herbert Boyer (1936–) created the first recombinant DNA organism, by adding recombinant plasmids to E. coli. Since that time, advances in molecular biology techniques, in particular the development of the polymerase chain reaction, have made the construction of recombinant DNA swifter and easier.
Recombinant DNA has been of fundamental importance in furthering the understanding of genetic regulatory processes and shows great potential in the genetic design of therapeutic strategies.
However, recombinant DNA has a darker side. It is conceivable that the genes specifying disease-causing components such as the toxin produced by the bacterium that causes anthrax could be combined with antibiotic-resistance genes, to produce an anthrax bacterium that was very difficult to kill using antibiotic therapy. This scenario is not far fetched. During the 1950s and 1960s both the United States and Russia undertook similar research.