Inside the host cell the recombinant DNA undergoes replication; thus, a bacterial host will give rise to a colony of cells containing the cloned target gene. Various screening methods may be used to identify such colonies, enabling them to be selected and cultured. Gene cloning facilitates DNA sequencing; it also enables large quantities of a desired protein product to be produced (see expression vector): human insulin, for example, is now produced by bacteria containing the cloned insulin gene. See also positional cloning.
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