Southern blotting

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Southern blotting A technique for transferring single-stranded (denatured) DNA fragments from a gel used for electrophoresis to a nitrocellulose filter. A gel containing the denatured DNA fragments is placed between blotting paper and a cellulose nitrate filter is saturated with a buffer. DNA is elued from the gel on to the cellulose nitrate, to which it binds permanently after baking at 80°C for two hours. The technique is used in DNA finger-printing and genetic engineering, for testing DNA hybridization.

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Southern blotting

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Southern blotting