Sanger's reagent

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Sanger's reagent A solution of 1-fluoro-2–4-dinitrobenzene, used for the chromatographic detection and quantification of amino acids, peptides, and proteins. Its effectiveness is based on the reaction of the reagent with free alpha- and epsilon-amino groups to form yellow dinitrophenyl derivatives.

Sanger's reagent

views updated

Sanger's reagent A solution of 1-fluoro-2-4-dinitrobenzene that is used for the chromatographic detection and quantification of amino acids, peptides, and proteins. Its effectiveness is based on the reaction of the reagent with free alpha-and epsilon-amino groups to form yellow dinitrophenyl derivatives.