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immunoassay
immunoassay Any of various techniques that measure the amount of a particular substance by virtue of its binding antigenically to a specific antibody. In solid-phase immunoassay the specific antibody is attached to a solid supporting medium, such as a PVC sheet. The sample is added and any test antigens will bind to the antibody. A second antibody, specific for a different site on the antigen, is added. This carries a radioactive or fluorescent label, enabling its concentration, and thus that of the test antigen, to be determined by comparison with known standards. Variations on this technique include ELISA and Western blotting. The principle is also now employed with certain types of microarray.
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"immunoassay." A Dictionary of Biology. 2004. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. "immunoassay." A Dictionary of Biology. 2004. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O6-immunoassay.html "immunoassay." A Dictionary of Biology. 2004. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O6-immunoassay.html |
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immunoassay
immunoassay (im-yoo-noh-ass-ay) n. any of various techniques for determining the levels of antigen and antibody in a tissue. See radioimmunoassay.
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Cite this article
"immunoassay." A Dictionary of Nursing. 2008. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. "immunoassay." A Dictionary of Nursing. 2008. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O62-immunoassay.html "immunoassay." A Dictionary of Nursing. 2008. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O62-immunoassay.html |
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