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ELISA

ELISA (enzyme-linked immunosorbent assay) A sensitive technique (see immunoassay) for accurately determining the amount of protein or other antigen in a given sample by means of an enzyme-catalysed colour change. Antibody specific to the test protein is adsorbed onto a solid substrate, such as a PVC sheet, and a measured amount of the sample is added; all molecules of the test protein in the sample are bound by the antibody. A second antibody specific for a second site on the test protein is added; this is conjugated with an enzyme, which catalyses a colour change in the fourth reagent, added finally to the sheet. The colour change can be measured photometrically and compared against a standard curve to give the concentration of protein in the sample. ELISA is widely used for diagnostic and other purposes.

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"ELISA." A Dictionary of Biology. . Encyclopedia.com. 23 Aug. 2017 <http://www.encyclopedia.com>.

"ELISA." A Dictionary of Biology. . Encyclopedia.com. (August 23, 2017). http://www.encyclopedia.com/science/dictionaries-thesauruses-pictures-and-press-releases/elisa

"ELISA." A Dictionary of Biology. . Retrieved August 23, 2017 from Encyclopedia.com: http://www.encyclopedia.com/science/dictionaries-thesauruses-pictures-and-press-releases/elisa

ELISA

ELISA Enzyme‐linked immunosorbent assay; extremely sensitive and specific analytical technique using antibodies linked to an enzyme system to amplify sensitivity.

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"ELISA." A Dictionary of Food and Nutrition. . Encyclopedia.com. 23 Aug. 2017 <http://www.encyclopedia.com>.

"ELISA." A Dictionary of Food and Nutrition. . Encyclopedia.com. (August 23, 2017). http://www.encyclopedia.com/education/dictionaries-thesauruses-pictures-and-press-releases/elisa

"ELISA." A Dictionary of Food and Nutrition. . Retrieved August 23, 2017 from Encyclopedia.com: http://www.encyclopedia.com/education/dictionaries-thesauruses-pictures-and-press-releases/elisa

ELISA

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"ELISA." A Dictionary of Nursing. . Encyclopedia.com. 23 Aug. 2017 <http://www.encyclopedia.com>.

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"ELISA." A Dictionary of Nursing. . Retrieved August 23, 2017 from Encyclopedia.com: http://www.encyclopedia.com/caregiving/dictionaries-thesauruses-pictures-and-press-releases/elisa

ELISA

ELISA (ɪˈlaɪzə) Med. enzyme-linked immunosorbent assay

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"ELISA." The Oxford Dictionary of Abbreviations. . Encyclopedia.com. 23 Aug. 2017 <http://www.encyclopedia.com>.

"ELISA." The Oxford Dictionary of Abbreviations. . Encyclopedia.com. (August 23, 2017). http://www.encyclopedia.com/humanities/dictionaries-thesauruses-pictures-and-press-releases/elisa

"ELISA." The Oxford Dictionary of Abbreviations. . Retrieved August 23, 2017 from Encyclopedia.com: http://www.encyclopedia.com/humanities/dictionaries-thesauruses-pictures-and-press-releases/elisa