Enzyme Immunoassay
ENZYME IMMUNOASSAY
Enzyme immunoassay (EIA) is a test used to detect and quantify specific antigen-eliciting molecules involved in biological processes, specifically processes related to cancer and autoimmune disorders. EIA can be used on most types of biological samples, such as plasma, serum, urine, and cell extracts. In the assay, a plate is coated with a primary antibody, which recognizes the antigen of the target molecule and bonds with it. The antigen-antibody complex is recognized by a secondary antibody that is joined to an enzyme that catalyzes the reaction mixture, yielding a specific color. By measuring the optical density of this color, the presence and number of a specific molecule can be determined; the density of color is proportional to the advancement of the reaction or disease being tested.
Mukesh Verma
Sudhir K. Srivastava
(see also: Antibody, Antigen )
More From encyclopedia.com
Specific Yield , specific yield Ribozyme , Ribozymes are RNA molecules that catalyze chemical reactions. Most biological processes do not happen spontaneously. For example, the cleavage of a m… Immunoassay , Definition
Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body fluid sample using an imm… Allergy Tests , Definition
Allergy tests evaluate levels of allergic sensitivity to commonly encountered allergens, which may be foods, pollen, chemicals, or other s… Warning Coloration , warning coloration (aposematic coloration) The conspicuous markings of an animal that make it easily recognizable and warn would-be predators that it… Color Therapy , Chromotherapy, the practice of healing with color, emerged in the nineteenth century as the object of scientific speculation and research, out of whi…
You Might Also Like
NEARBY TERMS
Enzyme Immunoassay