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polymerase chain reaction

A Dictionary of Biology | 2004 | © A Dictionary of Biology 2004, originally published by Oxford University Press 2004. (Hide copyright information) Copyright

polymerase chain reaction (PCR) A technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. PCR is commonly employed as an alternative to gene cloning as a means of amplifying genetic material for DNA sequencing. The technique has also proved invaluable in forensic science, enabling amplification of minute traces of genetic material for DNA fingerprinting or for detecting microsatellite DNA. The two strands of the DNA are separated by heating and short sequences of a single DNA strand (primers) are added, together with a supply of free nucleotides and DNA polymerase obtained from a bacterium that can withstand extreme heat. In a series of heating and cooling cycles, the DNA sequence flanked by the primers doubles with each cycle and is thus rapidly amplified. See also randomly amplified polymorphic DNA.

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Encyclopedia entry from: Encyclopedia of Espionage, Intelligence, and Security Polymerase Chain Reaction (PCR) █ BRYAN R. COBB The Polymerase Chain Reaction, or PCR, refers to a widely used technique in molecular biology that has become quintessential in many aspects of DNA analysis with broad-based applications...
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