reverse genetics
reverse genetics Any approach to genetic investigation that aims to find the function for some known protein or gene. It contrasts with the more traditional forward genetics approach, in which an unknown gene is sought for a known function (identified by the effect of a mutation). For example, analysis of gene sequences reveals open reading frames, which are the hallmarks of functional genes (see reading frame). Reverse genetics methods can be used to discover the function of such genes. For example, the gene can be cloned, subjected to mutation, and then reinserted into the organism (e.g. a bacterium or yeast cell) to see what effect the mutation has on function. A similar approach can be taken starting with a protein of unknown function. The amino-acid sequence can be back-translated into genetic code, a DNA probe constructed for part of the DNA sequence, and the relevant gene selected from a DNA library of the organism.
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Hermann Muller , Muller, Hermann
Muller, Hermann
Geneticist 1890-1967
Hermann Joseph Muller was one of the founding members of the "fly lab" that was initiated by Tho… Genetics , genetics, scientific study of the mechanism of heredity. While Gregor Mendel first presented his findings on the statistical laws governing the trans… Pseudogenes , Pseudogenes are defective copies of functional genes. These may be partial or complete duplicates derived from polypeptide-encoding genes or RNA gene… Microbial Genetics , Microbial genetics is a branch of genetics concerned with the transmission of hereditary characters in microorganisms. Within the usual definition, m… Gene , The gene is the physical unit of heredity. For each physical trait—such as eye color, height, hair color—a person inherits two genes or two groups of… Mutation , A mutation is any change in genetic material that is passed on to the next generation. The process of acquiring change in genetic material forms the…
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reverse genetics