|
Globular tail fragment of myosin-V displaces vesicle-associated motor and blocks vesicle transport in squid nerve cell extracts.
The Biological Bulletin; 10/1/2002; Brown, Jeremiah R. Peacock-Villada, Elizabeth M. Langford, George M.; 787 words
; Myosin-V, an actin-based motor that is highly enriched in the ... filaments. Recent evidence suggests that the globular tail of myosin-V interacts with the microtubule-based motor, kinesin ... In this study we show that the globular tail fragment of myosin-V displaces native myosin-V from vesicles ...
Read more
|
|
Drosophila crinkled, Mutations of Which Disrupt Morphogenesis and Cause Lethality, Encodes Fly Myosin VIIA
Genetics; 11/1/2004; Kiehart, Daniel P; Franke, Josef D; Chee, Mark K; Montague, R A; Et al; 787 words
; ABSTRACT Myosin VIIs provide motor function for a wide ... in crinkled (ck) disrupt the Drosophila myosin VIIA heavy chain. The ck/myoVIIA protein ... homology model for the structure of the ck/myosin VIIA head that indicates myosin VIIAs ...
Read more
|
|
The Comparison of Ca^sup 2+^/CaM-Independent and Ca^sup 2+^/CaM-Dependent Phosphorylation of Myosin Light Chains by MLCK
Physiological Research; 11/1/2005; Tang, Z; Chen, H; Yang, J; Dai, S; Lin, Y; 787 words
; ... 2+^/calmodulin (CaM)-dependent phosphorylation of myosin (CDPM), by myosin light chain kinase (MLCK). It is also known that the ... in intracellular Ca^sup 2+^ and phosphorylation of myosin occurs within a short time under physiological conditions ...
Read more
|
|
Myosin Cross-Bridge Kinetics and the Mechanism of Catch
Biophysical Journal; 7/15/2007; Franke, Aaron S; Mooers, Susan U; Narayan, Srinivasa R; Siegman, Marion J; Butler, Thomas M; 787 words
; ... mini-titin, twitchin. The kinetic parameters of myosin cross-bridge turnover in permeabilized ... determined by single turnover measurements on myosin-bound ADP. Under isometric conditions ... Also, there is no measurable turnover of myosin-bound ADP during relaxation of catch force ...
Read more
|
|
Kinetic effects of myosin regulatory light chain phosphorylation on skeletal muscle contraction
Biophysical Journal; 7/1/2002; Davis, Julien S; Satorius, Colleen L; Epstein, Neal D; 787 words
; ... actin-catalyzed release of phosphate from myosin. This step appears to be separate from ... how the interaction of the two heads of myosin could account for the inverse effect of ... dumbbell-shaped regulatory light chain (RLC) of myosin wraps around and stabilizes a segment ...
Read more
|
|
Stability and Kinetic Properties of C5-Domain from Myosin Binding Protein C and its Mutants
Biophysical Journal; 2/15/2008; Guardiani, Carlo; Cecconi, Fabio; Livi, Roberto; 787 words
; ... impact of three mutations of domain C5 from myosin binding protein C, correlated to Familial ... most important of which are the β-myosin heavy chain, the cardiac troponin T, titin, and cardiac myosin binding protein C (MyBP-C) (2). MyBP-C ...
Read more
|
|
GTPase Rho is involved in myosin-II-mediated contraction of pseudo-contractile rings and transport of vesicles in extracts of clam oocytes.
The Biological Bulletin; 10/1/2002; Wollert, Torsten DePina, Ana S. Thompson, Reid F. Langford, George M.; 787 words
; ... cytoskeleton is via the Rho-ROK/Rho kinase-myosin phosphatase pathway (Fig. 1A). In this pathway, myosin light-chain phosphatase (MyoP) is phosphorylated ... 2). The net result is the activation of myosin-II and the sustained contraction of smooth ...
Read more
|
|
Localization of myosin on tubulovesicular organelles in the squid giant axon immuno-EM.
The Biological Bulletin; 10/1/1996; Tabb, Joel S. Harmon, Khary O. DePina, Ana S. Langford, George M.; 738 words
; ... of this study was to determine whether myosin V is associated with these organelles ... isomerase (7, 8). Immuno-EM was used to detect myosin V on the membranes of these ER vesicles ... study was generated in rabbit against myosin V (mol. wt. 196 kD) purified from squid ...
Read more
|
|
[Ca.sup.2+] effects on myosin-II-mediated contraction of pseudo-contractile rings and transport of vesicles in extracts of clam oocytes.
The Biological Bulletin; 10/1/2002; Wollert, Torsten DePina, Ana S. Thompson, Reid F. Langford, George M.; 787 words
; ... stimulated phosphorylation of the regulatory light chain of myosin-II by myosin light chain kinase (MLCK). Therefore, [Ca.sup.2+] serves ... both as a signal for cytokinesis and as a regulator of myosin-II activity. We studied cytokinesis in vitro by reconstituting ...
Read more
|
|
Role of Actin DNase-I-Binding Loop in Myosin Subfragment 1-Induced Polymerization of G-actin: Implications for the Mechanism of Polymerization
Biophysical Journal; 4/1/2005; Wawro, Barbara; Khaitlina, Sofia Yu; Galinska-Rakoczy, Agnieszka; Strzelecka-Golaszewska, Hanna; 787 words
; ... filaments, albeit at a lower rate than unmodified actin, by myosin subfragment 1 (S1) carrying the A1 essential light chain but ... McCammon, 2001). At low ionic strength, actin can be polymerized by myosin subfragments, heavy meromyosin, or subfragment 1 (S1). Although ...
Read more
|