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biuret test
biuret test A biochemical test to detect proteins in solution, named after the substance biuret (H2NCONHCONH2), which is formed when urea is heated. Sodium hydroxide is mixed with the test solution and drops of 1% copper(II) sulphate solution are then added slowly. A positive result is indicated by a violet ring, caused by the reaction of peptide bonds in the proteins or peptides. Such a result will not occur in the presence of free amino acids.
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"biuret test." A Dictionary of Biology. 2004. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. "biuret test." A Dictionary of Biology. 2004. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O6-biurettest.html "biuret test." A Dictionary of Biology. 2004. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O6-biurettest.html |
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biuret test
biuret test A chemical test for proteins based on the formation of a violet colour when copper sulphate in alkaline solution reacts with a peptide bond.
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DAVID A. BENDER. "biuret test." A Dictionary of Food and Nutrition. 2005. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. DAVID A. BENDER. "biuret test." A Dictionary of Food and Nutrition. 2005. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O39-biurettest.html DAVID A. BENDER. "biuret test." A Dictionary of Food and Nutrition. 2005. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O39-biurettest.html |
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