|
Formation and Destabilization of Actin Filaments with Tetramethylrhodamine-Modified Actin
Biophysical Journal; 8/1/2004; Kudryashov, Dmitry S; Phillips, Martin; Reisler, Emil; 7298 words
; ABSTRACT Actin labeling at Cys^sup 374^ with tethramethylrhodamine derivatives (TMR-actin) has been widely used for direct observation of ... treadmilling, as well as for the in vivo visualization of actin cytoskeleton. The advantage of TMR-actin is that ...
Read more
|
|
The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1(W)
Plant Cell; 8/1/2005; Michelot, Alphée; Guérin, Christophe; Huang, Shanjin; Ingouff, Mathieu; Et al; 10722 words
; The organization of actin filaments into large ordered structures ... processes. However, the mechanisms by which actin filament polymerization is initiated from the available pool of profilin-bound actin monomers remain unknown in plants. Because ...
Read more
|
|
Role of Actin DNase-I-Binding Loop in Myosin Subfragment 1-Induced Polymerization of G-actin: Implications for the Mechanism of Polymerization
Biophysical Journal; 4/1/2005; Wawro, Barbara; Khaitlina, Sofia Yu; Galinska-Rakoczy, Agnieszka; Strzelecka-Golaszewska, Hanna; 8655 words
; ABSTRACT Proteolytic cleavage of actin between Gly^sup 42^ and Val^sup 43^ within ... D-loop) abolishes the ability of Ca-G-actin to spontaneously polymerize in the presence of KCI. Here we show that such modified actin is assembled into filaments, albeit at ...
Read more
|
|
Glycolytic Enzyme Interactions with Yeast and Skeletal Muscle F-Actin
Biophysical Journal; 2/15/2006; Waingeh, Victor F; Gustafson, Carol D; Kozliak, Evguenii I; Lowe, Stephen L; Et al; 9616 words
; ... Interaction of glycolytic enzymes with F-actin is suggested to be a mechanism for compartmentation ... Earlier work demonstrates that muscle F-actin strongly binds glycolytic enzymes, allowing for the general conclusion that "actin binds enzymes", which may be a generalized ...
Read more
|
|
Differential Interaction of Cardiac, Skeletal Muscle, and Yeast Tropomyosins with Fluorescent (Pyrene^sup 235^) Yeast Actin
Biophysical Journal; 2/15/2006; Chen, Weizu; Wen, Kuo-Kuang; Sens, Ashley E; Rubenstein, Peter A; 7609 words
; ... To monitor binding of tropomyosin to yeast actin, we mutated S^sup 235^ to C and labeled the actin with pyrene maleimide at both C^sup 235^ and ... pyrene fluorescence of the doubly labeled F-actin but no change in WT actin C^sup 374^ probe ...
Read more
|
|
Real-Time Measurements of Actin Filament Polymerization by Total Internal Reflection Fluorescence Microscopy
Biophysical Journal; 2/1/2005; Kuhn, Jeffrey R; Pollard, Thomas D; 10585 words
; ABSTRACT Understanding the mechanism of actin polymerization and its regulation by associated ... fluorescence signal is fourfold stronger with actin labeled on Cys-374 with Oregon green rather ... We estimated association rates at high actin concentrations and dissociation rates near ...
Read more
|
|
Solution properties of tetramethylrhodamine-modified G-actin
Biophysical Journal; 10/1/2003; Kudryashov, Dmitry S; Reisler, Emil; 6691 words
; ... solved structure of TMR-modified ADP-G-actin, the nucleotide cleft is in a closed state ... 374^ in determining these aspects of G-actin structure. We show here that the susceptibility of D-loop on G-actin to subtilisin cleavage, and ATP/ADP-dependent ...
Read more
|
|
Arabidopsis VILLIN1 Generates Actin Filament Cables That Are Resistant to Depolymerization
Plant Cell; 2/1/2005; Huang, Shanjin; Robinson, Robert C; Gao, Lisa Y; Matsumoto, Tracie; Et al; 11403 words
; ... migration use molecular tracks generated from actin filaments arrayed into higher-order structures like actin cables and bundles. How these arrays are ... Villin and fimbrin are the best characterized actin-filament bundling or cross-linking proteins ...
Read more
|
|
Atomic force microscopy and light scattering of small unilamellar actin-containing liposomes
Biophysical Journal; 8/1/2003; Palmer, Andre F; Wingert, Philip; Nickels, Jonathan; 9690 words
; ABSTRACT Three-dimensional networks of filamentous actin (F-actin) encapsulated inside phosphatidylcholine liposomes are ... yolk-derived phosphatidylcholine encapsulating monomeric actin (G-actin) were made via extrusion in low ionic strength ...
Read more
|
|
Large-Scale Quantitative Analysis of Sources of Variation in the Actin Polymerization-Based Movement of Listeria monocytogenes
Biophysical Journal; 7/1/2005; Soo, Frederick S; Theriot, Julie A; 12955 words
; ... models have been proposed in which change in the actin gel density (18) and actin filament density (17) have varying effects on bacterial movement. Although we cannot measure absolute actin density, we are able to accurately measure relative actin density in our high-throughput system as a ...
Read more
|