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gel filtration

A Dictionary of Biology | 2004 | © A Dictionary of Biology 2004, originally published by Oxford University Press 2004. (Hide copyright information) Copyright

gel filtration A type of column chromatography in which a mixture of liquids is passed down a column containing a gel. Small molecules in the mixture can enter pores in the gel and move slowly down the column; large molecules, which cannot enter the pores, move more quickly. Thus, mixtures of molecules can be separated on the basis of their size. The technique is used particularly for separating proteins but it can also be applied to cell nuclei, viruses, etc.

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