Rat Esophagus

Z projection of 8 slices through the rat esophagus tissue stained with Acridine Orange. Two-photon fluorescence (red) and SHG (green) visualize cell nuclei and connective tissue, respectively. A GRIN a diameter of 0.5 mm and a NA of 0.5. The lens used for imaging has excitation power on the sample resulting in two-photon fluorescence and SHG signals is 10 mW and 25 mW, respectively. slice spacing is 5 µm. Scale bar represents 20 µm. from the research of: Ling Fu, Ankur Jain, Huikai Xie, Charles Cranfield, and Min Gu