卵巢功能~~差~~的~~救星~~coQ10~~？

卵巢功能差的救星coQ10？dor Co-enzyme Q10 supplementation improves ovarian response and mitochondrial function in aged mice E. Burstein, A. Perumalsamy, Y. Bentov, N. Esfandiari, A. Jurisicova, R.F. Casper O-105 Tuesday, October 20, 2009 5:00 PM Article Outline • Copyright OBJECTIVE: Increased maternal age is accompanied by changes in metabolic activity of oocytes. As mitochondria are exclusively maternally inherited, alterations in their activity may severely compromise future embryo development. The objective was to compare the effects of maternal treatment with CoQ10, resveratrol or R-alpha lipoic acid (ALA), known mitochondrial nutrients, on MMP, reactive oxygen species (ROS) and mitochondrial DNA copy number in oocytes from aged ICR mice with placebo treated (aged control) or 10 week old (young control) mice. DESIGN: Randomized placebo-controlled in-vivo study. MATERIALS AND METHODS: Retired breeder (8 month old) ICR female mice (n = 40) were randomized to receive coQ10, resveratrol, R-ALA or vehicle alone via SC injections for the period of 18 weeks. Upon completion of treatment, mice were subjected to superovulation with gonadotropins, and oocytes were analyzed for mitochondrial membrane potential (JC1) and accumulation of ROS (H2DCFDA). The fluorescence of both dyes was analyzed using a deconvolution microscope and fluorescent signal was quantitated using the Delta Vision software (Silicon Graphics). Absolute mitochondrial DNA copy number was obtained by real-time PCR. All values were compared to oocytes obtained from young mice. RESULTS: Compared to aged controls, CoQ10 treatment significantly increased the number of ovulated oocytes (11.7 vs. 19.2) while R-ALA and resveratrol had no significant effect. Oocyte MMP (JC1 red to green ratio) was reduced and ROS levels were significantly increased by coQ10 treatment to levels comparable to those detected in the young oocytes. These parameters were not affected by the treatment with resveratrol or R-ALA. In addition, mitochondrial DNA copy number was elevated in old mice, CoQ10 treated females oocytes had lower mitochondrial copy number albeit still significantly higher than those found in the young oocytes (p]0.05). CONCLUSIONS: CoQ10 supplementation appears to improve ovarian response and oocyte mitochondrial function in old miceHow Dose Coenzyme Q10 Supplementation During Infertility Treatment Effects Pregnancy Outcome (CoQ10) This study is not yet open for participant recruitment. Verified by University of Toronto, April 2009 First Received: April 7, 2009 No Changes Posted Sponsors and Collaborators:University of Toronto The Toronto Center for Advanced Reproductive Technology (TCART) Ferring Pharmaceuticals Information provided by:University of Toronto ClinicalTrials.gov Identifier:NCT00878124 Purpose As women age the ovarian response and pregnancy rate are reduced while the rate of chromosomal abnormalities in the embryos is increased. Oocyte maturation, accurate chromosomal segregation as well as early embryo development and implantation are dependent on the supply of large amounts of energy, which unlike other cells can only arise from the mitochondria. With age the mitochondria becomes less efficient due to damage to its unique DNA, resulting in decreased energy production. The proposed study will examine the effect of the combination of a dietary supplement, Co enzyme Q10 on pregnancy rate and response of the ovaries to a medication that stimulates follicle growth. The investigators hypothesize that the improved energetic state of the oocyte would result in a more accurate chromosomal separation, increased embryo quality and pregnancy rate.