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gel filtration
gel filtration A column-chromatographic technique that normally employs as a stationary phase polymeric carbohydrate-gel beads of controlled size and porosity. Mixture components are separated on the basis of their sizes and rates of diffusion into the beads. Smaller molecules tend to diffuse more rapidly into the beads, thereby leaving the mainstream of solvent and so becoming retarded with respect to larger molecules. This method can also be used to determine the molecular weight of an unknown substance.
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MICHAEL ALLABY. "gel filtration." A Dictionary of Zoology. 1999. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. MICHAEL ALLABY. "gel filtration." A Dictionary of Zoology. 1999. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O8-gelfiltration.html MICHAEL ALLABY. "gel filtration." A Dictionary of Zoology. 1999. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O8-gelfiltration.html |
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gel filtration
gel filtration A column-chromatographic technique normally employing as a stationary phase polymeric carbohydrate-gel beads of controlled size and porosity. Mixture components are separated on the basis of their sizes and rates of diffusion into the beads. Smaller molecules tend to diffuse more rapidly into the beads, thereby leaving the mainstream of solvent and so becoming retarded with respect to larger molecules. This method can also be used to determine the molecular weight of an unknown substance.
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Cite this article
MICHAEL ALLABY. "gel filtration." A Dictionary of Plant Sciences. 1998. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. MICHAEL ALLABY. "gel filtration." A Dictionary of Plant Sciences. 1998. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O7-gelfiltration.html MICHAEL ALLABY. "gel filtration." A Dictionary of Plant Sciences. 1998. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O7-gelfiltration.html |
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gel filtration
gel filtration A column chromatographic technique normally employing as a stationary phase polymeric carbohydrategel beads of controlled size and porosity. Mixture components are separated on the basis of their sizes and rates of diffusion into the beads. Smaller molecules tend to diffuse more rapidly into the beads, thereby leaving the mainstream of solvent and so becoming retarded with respect to larger molecules. This method can also be used to determine the molecular weight of an unknown substance.
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Cite this article
MICHAEL ALLABY. "gel filtration." A Dictionary of Ecology. 2004. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. MICHAEL ALLABY. "gel filtration." A Dictionary of Ecology. 2004. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O14-gelfiltration.html MICHAEL ALLABY. "gel filtration." A Dictionary of Ecology. 2004. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O14-gelfiltration.html |
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gel filtration
gel filtration A type of column chromatography in which a mixture of liquids is passed down a column containing a gel. Small molecules in the mixture can enter pores in the gel and move slowly down the column; large molecules, which cannot enter the pores, move more quickly. Thus, mixtures of molecules can be separated on the basis of their size. The technique is used particularly for separating proteins but it can also be applied to cell nuclei, viruses, etc.
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Cite this article
"gel filtration." A Dictionary of Biology. 2004. Encyclopedia.com. 31 May. 2012 <http://www.encyclopedia.com>. "gel filtration." A Dictionary of Biology. 2004. Encyclopedia.com. (May 31, 2012). http://www.encyclopedia.com/doc/1O6-gelfiltration.html "gel filtration." A Dictionary of Biology. 2004. Retrieved May 31, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O6-gelfiltration.html |
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