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The Structure of Isolated Cardiac Myosin Thick Filaments from Cardiac Myosin Binding Protein-C Knockout Mice
Biophysical Journal; 3/1/2008; Kensler, Robert W; Harris, Samantha P; 8731 words
; ABSTRACT Mutations in the thick filament associated protein cardiac myosin binding ... this protein in stability of the thick filament and maintenance of the ordered helical ... questions, the structure of myosin thick filaments isolated from the hearts of wild-type ...
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Kinetics of the Formation and Dissociation of Actin Filament Branches Mediated by Arp2/3 Complex
Biophysical Journal; 11/1/2006; Mahaffy, Rachel E; Pollard, Thomas D; 6557 words
; ABSTRACT The actin filament network at the leading edge of motile ... branching by Arp2/3 complex from "mother" filaments growing near the plasma membrane. The nucleotide bound to the mother filaments (ATP, ADP and phosphate, or ADP) may influence ...
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Real-Time Measurements of Actin Filament Polymerization by Total Internal Reflection Fluorescence Microscopy
Biophysical Journal; 2/1/2005; Kuhn, Jeffrey R; Pollard, Thomas D; 10585 words
; ... to monitor polymerization dynamics and filament topology simultaneously. The only assay ... N-ethylmaleimide inactivated myosins attach filaments to the glass coverslip. We estimated ... unpolymerized monomers, initiate new filaments, cap filament ends, and promote filament ...
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Multiple structures of thick filaments in resting cardiac muscle and their influence on cross-bridge interactions
Biophysical Journal; 8/1/2001; Levine, Rhea; Weisberg, Andrea; Kulikovskaya, Irina; McClellan, L George; Winegrad, Saul; 6247 words
; ... myosin rods within the backbone of the filament and the degree of order of the myosin heads, thick filaments isolated from a control group of rat ... structures. Two of the structures of thick filaments had ordered myosin heads and were distinguishable ...
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Mammalian cardiac muscle thick filaments: Their periodicity and interactions with actin
Biophysical Journal; 3/1/2002; Kensler, Robert W; 7638 words
; ... available on the detailed macromolecular structure of its thick filament. To elucidate the structure of these filaments I have developed a procedure to isolate the cardiac thick filaments for study by electron microscopy and computer image analysis ...
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Measurement of thin filament lengths by distributed deconvolution analysis of fluorescence images
Biophysical Journal; 5/1/2002; Littlefield, Ryan; Fowler, Velia M; 10824 words
; ABSTRACT The lengths of the actin (thin) filaments in sarcomeres directly influence the ... precision and accuracy for measuring thin filament lengths, significant obstacles limit ... determines the location of specific thin filament components such as tropomodulin (Tmod ...
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Role of Ca2+ and cross-bridges in skeletal muscle thin filament activation probed with Ca2+ sensitizers
Biophysical Journal; 4/1/1999; Wahr, Philip A; Metzger, Joseph M; 7108 words
; ABSTRACT Thin filament regulation of contraction is thought ... cross-bridge states required to promote thin filament activation have not been identified ... between cross-bridge cycling and thin filament activation by comparing the results ...
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Bacterial flagellar microhydrodynamics: Laminar flow over complex flagellar filaments, analog archimedean screws and cylinders, and its perturbations
Biophysical Journal; 9/1/2003; Trachtenberg, Shlomo; Fishelov, Dalia; Ben-Artzi, Matania; 5104 words
; ... microscopy and helical image reconstruction methods, we calculated three-dimensional density maps of the complex filament of Rhizobium lupini H13-3 and determined its surface pattern and boundaries ... filament. The perturbation, or, rather, its structural effect, may, then, propagate along the ...
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Biochemical and Genetic Analyses Provide Insight Into the Structural and Mechanistic Properties of Actin Filament Disassembly by the Aip1p-Cofilin Complex in Saccharomyces cerevisiae
Genetics; 7/1/2007; Clark, Michael G; Amberg, David C; 8911 words
; ... Explication of the Aip1p/cofilin/actin filament complex may lead to a more detailed understanding ... cofilin collaborate to rapidly disassemble filaments. We further characterized the actin-Aip1p ... intersubunit contacts within an actin filament. In addition, site-directed mutagenesis ...
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Activation of the calcium-regulated thin filament by myosin strong binding
Biophysical Journal; 10/1/2003; Gorga, Joseph A; Fishbaugher, David E; VanBuren, Peter; 4814 words
; ... move at slower velocities independent of filament length indicates that the reduction in ... likely the rate-limiting step for thin filament motility at maximal calcium activation ... cooperative behavior in the regulated actin filament during actin activated ATP hydrolysis in ... by strong binding of myosin ...
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