Share this Article on Facebook Twitter Submit this article to Reddit Give this article a thumbs-up on StumbleUpon

Southern blotting

Southern blotting

A Dictionary of Biology | 2004 | Copyright

Southern blotting A chromatographic technique for isolating and identifying specific fragments of DNA, such as the fragments formed as a result of DNA cleavage by restriction enzymes. The mixture of fragments is subjected to electrophoresis through an agarose gel, followed by denaturation to form single-stranded fragments. These are transferred, or `blotted', onto a nitrocellulose filter where they are immobilized in their relative positions. Specific gene probes labelled with a radioisotope are then added. These hybridize with any complementary fragments on the filter, which are subsequently revealed by autoradiography. The technique was devised by US biologist E. M. Southern (1938– ). A similar technique for detecting RNA fragments is called Northern blotting, by analogy. See also Western blotting.
Show all research tools

Cite this article
Pick a style below, and copy the text for your bibliography.

  • MLA
  • Chicago
  • APA

"Southern blotting." A Dictionary of Biology. 2004. Encyclopedia.com. 1 Jun. 2012 <http://www.encyclopedia.com>.

"Southern blotting." A Dictionary of Biology. 2004. Encyclopedia.com. (June 1, 2012). http://www.encyclopedia.com/doc/1O6-Southernblotting.html

"Southern blotting." A Dictionary of Biology. 2004. Retrieved June 01, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O6-Southernblotting.html

Learn more about citation styles

Southern blotting

A Dictionary of Zoology | 1999 | | Copyright

Southern blotting A technique for transferring single-stranded (denatured) DNA fragments from a gel used for electrophoresis to a nitrocellulose filter. A gel containing the denatured DNA fragments is placed between blotting paper and a cellulose nitrate filter is saturated with a buffer. DNA is elued from the gel on to the cellulose nitrate, to which it binds permanently after baking at 80°C for two hours. The technique is used in DNA finger-printing and genetic engineering, for testing DNA hybridization.
Show all research tools

Cite this article
Pick a style below, and copy the text for your bibliography.

  • MLA
  • Chicago
  • APA

MICHAEL ALLABY. "Southern blotting." A Dictionary of Zoology. 1999. Encyclopedia.com. 1 Jun. 2012 <http://www.encyclopedia.com>.

MICHAEL ALLABY. "Southern blotting." A Dictionary of Zoology. 1999. Encyclopedia.com. (June 1, 2012). http://www.encyclopedia.com/doc/1O8-Southernblotting.html

MICHAEL ALLABY. "Southern blotting." A Dictionary of Zoology. 1999. Retrieved June 01, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O8-Southernblotting.html

Learn more about citation styles

Free newspaper and magazine articles

Better blotting. (Processing).(Brief Article)
Magazine article from: Food Engineering &amp; Ingredients; 6/1/2003
Large capacity hybridization oven.(Blotting, Gel Analysis Focus)
Magazine article from: Bioscience Technology; 11/1/2007
Use of methylation sensitive polymerase chain reaction for detection of...
Magazine article from: Indian Journal of Medical Research; 11/1/2005

Pictures from Google Image Search

Click to see an enlarged picture
Click to see an enlarged picture
Click to see an enlarged picture

See more pictures of Southern blotting

Related topics

  Be the first to connect related topics with this page!