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ELISA
ELISA (enzyme-linked immunosorbent assay) A sensitive technique (see immunoassay) for accurately determining the amount of protein or other antigen in a given sample by means of an enzyme-catalysed colour change. Antibody specific to the test protein is adsorbed onto a solid substrate, such as a PVC sheet, and a measured amount of the sample is added; all molecules of the test protein in the sample are bound by the antibody. A second antibody specific for a second site on the test protein is added; this is conjugated with an enzyme, which catalyses a colour change in the fourth reagent, added finally to the sheet. The colour change can be measured photometrically and compared against a standard curve to give the concentration of protein in the sample. ELISA is widely used for diagnostic and other purposes.
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"ELISA." A Dictionary of Biology. 2004. Encyclopedia.com. 11 Feb. 2012 <http://www.encyclopedia.com>. "ELISA." A Dictionary of Biology. 2004. Encyclopedia.com. (February 11, 2012). http://www.encyclopedia.com/doc/1O6-ELISA.html "ELISA." A Dictionary of Biology. 2004. Retrieved February 11, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O6-ELISA.html |
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ELISA
ELISA Enzyme‐linked immunosorbent assay; extremely sensitive and specific analytical technique using antibodies linked to an enzyme system to amplify sensitivity.
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DAVID A. BENDER. "ELISA." A Dictionary of Food and Nutrition. 2005. Encyclopedia.com. 11 Feb. 2012 <http://www.encyclopedia.com>. DAVID A. BENDER. "ELISA." A Dictionary of Food and Nutrition. 2005. Encyclopedia.com. (February 11, 2012). http://www.encyclopedia.com/doc/1O39-ELISA.html DAVID A. BENDER. "ELISA." A Dictionary of Food and Nutrition. 2005. Retrieved February 11, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O39-ELISA.html |
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ELISA
ELISA n. see enzyme-linked immunosorbent assay.
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"ELISA." A Dictionary of Nursing. 2008. Encyclopedia.com. 11 Feb. 2012 <http://www.encyclopedia.com>. "ELISA." A Dictionary of Nursing. 2008. Encyclopedia.com. (February 11, 2012). http://www.encyclopedia.com/doc/1O62-ELISA.html "ELISA." A Dictionary of Nursing. 2008. Retrieved February 11, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O62-ELISA.html |
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ELISA
ELISA (ɪˈlaɪzə) Med. enzyme-linked immunosorbent assay
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Cite this article
FRAN ALEXANDER , PETER BLAIR , JOHN DAINTITH , ALICE GRANDISON , VALERIE ILLINGWORTH , ELIZABETH MARTIN , ANNE STIBBS , JUDY PEARSALL , and SARA TULLOCH. "ELISA." The Oxford Dictionary of Abbreviations. 1998. Encyclopedia.com. 11 Feb. 2012 <http://www.encyclopedia.com>. FRAN ALEXANDER , PETER BLAIR , JOHN DAINTITH , ALICE GRANDISON , VALERIE ILLINGWORTH , ELIZABETH MARTIN , ANNE STIBBS , JUDY PEARSALL , and SARA TULLOCH. "ELISA." The Oxford Dictionary of Abbreviations. 1998. Encyclopedia.com. (February 11, 2012). http://www.encyclopedia.com/doc/1O25-ELISA.html FRAN ALEXANDER , PETER BLAIR , JOHN DAINTITH , ALICE GRANDISON , VALERIE ILLINGWORTH , ELIZABETH MARTIN , ANNE STIBBS , JUDY PEARSALL , and SARA TULLOCH. "ELISA." The Oxford Dictionary of Abbreviations. 1998. Retrieved February 11, 2012 from Encyclopedia.com: http://www.encyclopedia.com/doc/1O25-ELISA.html |
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