thermal denaturation

A Dictionary of Biology

thermal denaturation See denature.
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© A Dictionary of Biology 2004, originally published by Oxford University Press 2004.

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	Exceptional sensitivity of rubisco activase to thermal denaturation in vitro and in vivo ; ...Sensitivity of Rubisco Activase to Thermal Denaturation in Vitro and in Vivo1 Heat stress...of these experiments showed that thermal denaturation of activase in vivo occurred at...exceptional sensitivity of the protein to thermal denaturation and is responsible, in part, for... Read more
	Reversible thermal denaturation of human FGF-1 induced by low concentrations of guanidine hydrochloride ; ...presumably due to effects of irreversible thermal denaturation. Here we report the determination...scanning calorimetry (DSC). The thermal denaturation is demonstrated to be two-state...protection of FGF-1 by heparin against thermal denaturation and proteolysis. The low T^sub m... Read more
	Thermal Unfolding Simulations of Bacterial Flagellin: Insight into its Refolding Before Assembly ; ...domains as reported in experimental thermal denaturation. D2a and D3 exhibited high thermal...flagellin-folding mechanism via thermal denaturation was carried out by Honda et al...affect the unfolding pathway, as thermal denaturation is an activaied process where lower... Read more
	[beta]-Lactoglobulin is a Thermal Marker in Processed Milk as Studied by Electrophoresis and Circular Dichroic Spectra ; ...2-dimensional PAGE. The detailed thermal denaturation of purified LG and LA at various...processing of milk. The detailed thermal denaturation curves of LG constructed with its...946;-lactoglobulin structure, thermal denaturation, processed milk, α-lactalbumin... Read more
	Thermal and Sodium Dodecylsulfate Induced Transitions of Streptavidin ; ...is known about the nature of the thermal denaturation pathway for SA. This work uses a...structurally quite stable with respect to thermal denaturation or melting (Bayer et al., 1990...structural changes accompanying thermal denaturation, though a full picture of the thermal... Read more
	The Kinetics of Heat-Induced Structural Changes of [beta]-Lactoglobulin ; ...involved in this complex process. The thermal denaturation of β-LG was found to be complex...There are numerous reports on the thermal denaturation of β-LG in buffer solutions...Manderson et al., 1999). Although thermal denaturation of β-LG clearly involves some... Read more
	Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal Denaturation ; ...65C. In this research, the sequential events in ACYI thermal denaturation were investigated by a combination of spectroscopic methods...be regarded as a thermostable enzyme. In general, the thermal denaturation of proteins can be demonstrated by a two-state thermodynamic... Read more
	Methionine Adenosyltransferase [alpha]-Helix Structure Unfolds at Lower Temperatures than [beta]-Sheet: A 2D-IR Study ; ...enzyme by fitting the amide I band of infrared spectra. Thermal denaturation studies allow the identification of events associated...enzyme activity and intrinsic fluorescence. In all cases, thermal denaturation proved to be an irreversible process, with a T^sub m... Read more
	Conformational Changes in Azurin from Pseudomona aeruginosa Induced through Chemical and Physical Protocols ; ...properties of the macromolecule are changed. The thermal denaturation of azurin immobilized in a sol-gel monolith...chemical denaturants (or stabilizers), upon thermal denaturation or upon immobilizing the protein in porous... Read more
	Fourier transform raman study of the structural specificities on the interaction between DNA and biogenic polyamines ; ...series of experimental evidences, such as precipitation, thermal denaturation, or protection. However, binding between polyamines and...polyamines on dsDNA were related to precipitation and thermal denaturation (Tabor and Tabor, 1984). A first molecular interpretation... Read more

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denature ...its biological properties. Denaturation involves unfolding of the polypeptide...structure; it is caused by heat (thermal denaturation), chemicals, and extremes of...eggs are largely a result of denaturation. Compare renaturation . Read more

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