Glutamine Synthetase-Glutamate Synthase Pathway and Glutamate Dehydrogenase Play Distinct Roles in the Sink-Source Nitrogen Cycle in Tobacco1

From: Plant Physiology | Date: February 1, 2006| Author: Masclaux-Daubresse, Cà © line; Reisdorf-Cren, Michà ¨ le; Pageau, Karine; Lelandais, Maud; Et al | Copyright information

Glutamate (Glu) metabolism and amino acid translocation were investigated in the young and old leaves of tobacco (Nicotiana tabacum L. cv Xanthi) using [^sup 15^N]ammonium and [2-^sup 15^N]Glu tracers. Regardless of leaf age, [^sup 15^N]ammonium assimilation occurred via glutamine synthetase (GS; EC 6.1.1.3) and Glu synthase (ferredoxin [Fd]-GOGAT; EC 1.4.7.1; NADH-GOGAT; EC 1.4.1.14), both in the light and darkness, and it did not depend on Glu dehydrogenase (GDH; EC 1.4.1.2). The [^sup 15^N...

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