Rapid assay for detection of chloramphenicol acetyl transferase in Haemophilus influenzae

Indian Journal of Medical Research | August 1, 2001| | Copyright

Background & objectives: Haemophilus influenzae causes a variety of life threatening infections in humans. Early detection of antimicrobial resistance is of importance in the treatment and management of infection. The modified Slack's method, a simple assay, has been evaluated in this study for the early detection of chloramphenicol resistance.

Methods: Fifty isolates of H. influenzae from invasive and non-invasive sites were included. Antimicrobial susceptibility testing was done by disc diffusion method and minimum inhibitory concentration (MIC) determination was performed for ...

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