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Species-Specific Differences in Mouse and Human Airway Epithelial Biology of Recombinant Adeno-Associated Virus Transduction
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Differences in airway epithelial biology between mice and humans have presented challenges to evaluating gene therapies for cystic fibrosis (CF) using murine models. In this context, recombinant adeno-associated virus (rAAV) type 2 and rAAV5 vectors have very different transduction efficiencies in human air-liquid interface (ALI) airway epithelia (rAAV2 = rAAV5) as compared with mouse lung (rAAVS [much greater than] rAAV2). It is unclear if these differences are due to species-specific airway...
Related newspaper, magazine, and journal articles from HighBeam Research
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Species-Specific Differences in Mouse and Human Airway Epithelial Biology of Recombinant Adeno-Associated Virus Transduction
American Journal of Respiratory Cell and Molecular Biology
; Differences in airway epithelial biology between mice and humans have presented challenges to evaluating gene therapies for cystic fibrosis (CF) using murine models. In this context, recombinant adeno-associated virus (rAAV) type 2 and rAAV5 vectors have very different transduction efficiencies in
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Bioelectric Properties of Chloride Channels in Human, Pig, Ferret, and Mouse Airway Epithelia
American Journal of Respiratory Cell and Molecular Biology
; The development of effective therapies for cystic fibrosis (CF) requires animal models that can appropriately reproduce the human disease phenotype. CF mouse models have demonstrated cAMP-inducible, non-CF transmembrane conductance regulator (non-CFTR) chloride transport in conducting airway
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Thixotropic solutions enhance viral-mediated gene transfer to airway epithelia
American Journal of Respiratory Cell and Molecular Biology
; Adenovirus-mediated gene transfer to airway epithelia is inefficient in part because its receptor is absent on the apical surface of the airways. Targeting adenovirus to other receptors, increasing the viral concentration, and even prolonging the incubation time with adenovirus vectors can
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Regulated Hydrogen Peroxide Production by Duox in Human Airway Epithelial Cells
American Journal of Respiratory Cell and Molecular Biology
; Hydrogen peroxide (H^sub 2^O^sub 2^) is found in exhaled breath and is produced by airway epithelia. In addition, H^sub 2^O^sub 2^ is a necessary substrate for the airway lactoperoxidase (LPO) anti-infection system. To investigate the source of H^sub 2^O^sub 2^ produced by airway epithelia, PCR was
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Long-Term Cultures of Polarized Airway Epithelial Cells from Patients with Cystic Fibrosis
American Journal of Respiratory Cell and Molecular Biology
; The poor ability of respiratory epithelial cells to proliferate and differentiate in vitro into a pseudostratified mucociliated epithelium limits the general use of primary airway epithelial cell (AEC) cultures generated from patients with rare diseases, such as cystic fibrosis (CF). Here, we
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CCL20 is an inducible product of human airway epithelia with innate immune properties
American Journal of Respiratory Cell and Molecular Biology
; Chemokine ligand 20 (CCL20) and human [beta]-defensins (HBDs) share structural and functional properties, including antiparallel [beta]-pleated sheet core structures, charge distribution, and signaling to adaptive immune cells via the highly selective CCR6 receptor. Because of their similarities,
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Human airway epithelia express a beta-defensin
American Journal of Respiratory Cell and Molecular Biology
; Recent evidence suggests that the surface fluid secreted by human airway epithelial cells contains antimicrobial activity. The identification of the factor(s) responsible for the antibacterial activity in airway surface fluid may bring new insights into lung defense mechanisms and the pathogenesis
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Interleukin-9 induces goblet cell hyperplasia during repair of human airway epithelia
American Journal of Respiratory Cell and Molecular Biology
; Asthma is characterized by airway inflammation, smooth muscle hyperreactivity, and airway remodeling with excessive mucus production. The effect cytokines like interleukin (IL)-9 have on airway epithelia has been addressed using murine models of asthma, as well as transgenic and knockout mice.
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Haemophilus influenzae Forms Biofilms on Airway Epithelia: Implications in Cystic Fibrosis
American Journal of Respiratory and Critical Care Medicine
; Rationale: Nontypeable Haemophilus influenzae (NTHi) commonly infects patients with cystic fibrosis (CF), especially early in childhood. Bacteria biofilms are increasingly recognized as contributing to bacterial persistence and disease pathogenesis in CF. Objectives: This study investigated ability
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Erratum
American Journal of Respiratory Cell and Molecular Biology
; "Human Airway Epithelia Express a alpha-defensin" by Paul B. McCray, Jr. and Laura Bentley, which appeared in the March 1997 issue of the Journal, should have been published as a Rapid Communication in the January 1997 issue.
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