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Automated Spectrophotometric Analysis of Mitochondrial Respiratory Chain Complex Enzyme Activities in Cultured Skin Fibroblasts
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Background: Mitochondrial respiratory chain complex (RCC) disorders may occur as commonly as 1 in 8500 individuals. Because of the great variability of phenotypic presentations, measurement of individual RCC enzyme activities is a crucial diagnostic process. Current assay methods are time-consuming and labor-intensive and thus constitute a major impediment to clinical practice. A method with a faster turnaround time would therefore be beneficial.
Method: We developed an automated spectrophotometric method for measuring the respiratory chain enzyme activities of complex I, complex II + III, ...
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Oxygen modulates the growth of skin fibroblasts
; ...tensions are inhibitory to the growth of skin fibroblasts. Skin fibroblasts grow better at oxygen tensions below 137...range, strongly modulate the growth of human skin fibroblasts. There were no significant differences between...
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The effect of erythrodiol-3-acetate on the expressions of matrix metalloproteinase-1 and type-1 procollagen caused by ultraviolet irradiated cultured primary old aged human skin fibroblasts.
; ...inhibition in primary old aged human skin fibroblasts caused by ultraviolet (UV) irradiation...irradiated cultured old aged human skin fibroblasts. Taken together, our results suggest...procollagen expression in cultured human skin fibroblasts caused by UV irradiation. This paper...
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INFLUENCE OF SERUM ON ADULT AND FETAL DERMAL FIBROBLAST MIGRATION, ADHESION, AND COLLAGEN EXPRESSION
; ...adhesion, and collagen expression by skin fibroblasts. Dermal fibroblasts were isolated...greater fraction of adherent fetal skin fibroblasts than adult fibroblasts in serum-free...serum-containing medium, suggesting that fetal skin fibroblasts possess serum-independent adhesion...
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Optimized Spectrophotometric Assay for the Completely Activated Pyruvate Dehydrogenase Complex in Fibroblasts
; ...dehydrogenase complex (PDHc) activity in human skin fibroblasts is hampered by low enzyme activity...investigation of the enzyme complex in human skin fibroblasts. 2005 American Association for Clinical...than skin biopsies, cultured human skin fibroblasts are used more commonly than ...
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Mitochondrial NADH- or NADPH-linked aquacobalamin reductase activity is low in human skin fibroblasts with defects in synthesis of cobalamin coenzymes
; ...Reductase Activity Is Low in Human Skin Fibroblasts with Defects in Synthesis of Cobalamin...Cell culture. Human normal and mutant skin fibroblasts were grown in Dulbecco's modified...at 300 X g for 5 min. Human normal skin fibroblasts (OT-40 and WI-38) were kindly provided...
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Inhibitory effects of human serum on human fetal skin fibroblast migration: Migration-inhibitory activity and substances in serum, and its age-related changes
; ...suppressed the migration of human fetal skin fibroblasts into a denuded area in a cell monolayer...suppressed the migration of human fetal skin fibroblasts, although it stimulated cell proliferation...that the migratory ability of human skin fibroblasts declines with in vitro aging ...
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Direct Nonisotopic Assay of 3-Methylglutaconyl-CoA Hydratase in Cultured Human Skin Fibroblasts to Specifically Identify Patients with 3-Methylglutaconic Aciduria Type I
; ...measurement of residual 3MGH activity in cultured human skin fibroblasts. We obtained KH^sub 4^PO^sub 4^, H^sub 3^PO^sub 4^, Tris...bicinchoninic acid were purchased from Sigma. We obtained skin fibroblasts from 2 individuals with confirmed 3MGA type T (7, 6), 5...
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Inhibitory Effects of Mizolastine on Ultraviolet B-Induced Leukotriene B4 Production and 5-Lipoxygenase Expression in Normal Human Dermal Fibroblasts In Vitro
; ...fibroblasts. Therefore, primary human skin fibroblasts were incubated with mizolastine or...5-lipoxygenase (5-LOX) messenger RNA (mRNA) in skin fibroblasts was examined by reverse transcriptase-polymera...inhibited UVB-induced LTB4 production from skin fibroblasts at 12, 24 and 36 h. ...
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Disease-related Metabolites in Culture Medium of Fibroblasts from Patients with D-2-Hydroxyglutaric Aciduria, L-2-Hydroxyglutaric Aciduria, and Combined D/L-2-Hydroxyglutaric Aciduria
; ...investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes...studies are performed on cultured skin fibroblasts, followed by DNA/RNA mutational analysis...L-2-HG in media of cultured human skin fibroblasts taken from patients with ...
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Lysosomes are sites of fluoroquinolone photosensitization in human skin fibroblasts: A microspectrofluorometric approach
; ...Are Sites of Fluoroquinolone Photosensitization in Human Skin Fibroblasts: A Microspectrofluorometric Approach* ABSTRACT The fluoroquinolone...BAYy3118 are readily incorporated into lysosomes of HS68 human skin fibroblasts although weak staining of the whole cytoplasm also occurs...
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