Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs

From: Infectious Diseases in Obstetrics and Gynecology | Date: September 1, 2005| Author: | Copyright information

Abstract

Objective. To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection of Trichomonas vaginalis using self-collected vaginal swabs.

Methods. Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs. T. vaginalis culture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Real-Time PCR Detection of Parapoxvirus DNA
; ...practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses...binder-based quantitative real-time PCR assay targeting the B2L gene of...LightCycler platforms. Results: The real-time PCR assay successfully ...
Development of a 5'-Nuclease Real-Time PCR Assay Targeting fliP for the Rapid Identification of Burkholderia mallei in Clinical Samples
; ...mallei-specific 5'-nuclease real-time PCR assay that targets the fliP gene...PCR, our rapid 5'-nuclease real-time PCR assay for the specific identification...post-PCR manipulations. This real-time PCR assay also shortens the ...
MALDI-TOF Mass Spectrometry Compared With Real-Time PCR for Detection of Fetal Cell-Free DNA in Maternal Plasma
; ...MALDl-TOF MS assay and the TaqMan real-time PCR assay in parallel. The presence...95% (55/58), and the TaqMan real-time PCR assay yielded a sensitivity of W...negative according to the TaqMan real-time PCR assay. The 3 samples were obtained...
Development of an Assay to Quantify Rumen Ciliate Protozoal Biomass in Cows Using Real-Time PCR1
; ...report the development of a real-time PCR assay targeting the gene encoding...KEY WORDS: * rumen protozoa * real-time PCR * 18S rDNA * cattle Ciliate...research was to develop a real-time PCR assay to quantify protozoal biomass...
Detection of group B streptococci (GBS) in vaginal swabs using real-time PCR with TaqMan probe hybridization
; ...was to develop a real-time PCR method for the...constructed for a real-time PCR assay. The performance...conclusion: The real-time PCR assay was sensitive...streptococci (GBS) - real-time PCR - TaqMan probe...chain reaction ...
HER-2/neu gene copy number quantified by real-time PCR: Comparison of gene amplification, heterozygosity, and immunohistochemical status in breast cancer tissue
; ...We developed a rapid-cycle, real-time PCR assay for quantification of HER-2...HER-2/neu quantification by real-time PCR were 12% and 18%, and the CV...developed a rapid-cycle, real-time PCR assay (17) for HER-2/neu, evaluated...
Quantitative Real-Time PCR Assay for Rapid Identification of Deletion Carriers in Hemophilia
; ...diseases, but conventional PCR often fails to detect such...a carrier. We designed a real-time quantitative PCR assay to establish heterozygous...11p15) were amplified by real-time PCR from DNA obtained from the...status. The proposed rapid real-time ...
A Novel Real-Time Duplex PCR Assay for Detecting penA and ponA Genotypes in Neisseria gonorrhoeae: Comparison with Phenotypes Determined by the E-Test
; ...The real-time duplex PCR assay successfully detected...obtained with the real-time PCR and the melting analysis...recently validated real-time PCR assay (10) has been used...methods and a novel real-time PCR assay to ...
Development and assessment of a quantitative reverse transcription-PCR assay for simultaneous measurement of four amplicons
; ...transcription (QRT)-PCR assay for human radiation-responsi...smaller, less costly, real-time PCR detection system...a quadruplex QRT-PCR assay in a 96-well, closed-pla...With the advent of real-time PCR technology (1) and...improved quantitative PCR ...
Multiplexed Real-Time PCR Using Universal Reporters
; ...efforts. Here we present a real-time PCR assay that uses universal hybridization-bas...The principle of the cr-real-time PCR assay is as follows: Target DNA...detection. Because the cr-real-time PCR assay requires three interacting...