Fatty Acids Induce L-CPT I Gene Expression through a PPAR[alpha]-Independent Mechanism in Rat Hepatoma Cells

From: The Journal of Nutrition | Date: October 1, 2005| Author: | Copyright information

ABSTRACT

Liver carnitine palmitoyl transferase (L-CPT) I is a key regulatory enzyme of long-chain fatty acid (LCFA) oxidation that ensures the first step of LCFA import into the mitochondrial matrix. In rat hepatocytes, we showed previously that L-CPT I gene expression was induced by LCFAs as well as by fibrates. The aim of this study was to determine whether LCFA-induced L-CPT I gene expression was mediated by PPARα. For this purpose, we constructed a PPARα-dominant negative receptor to inhibit endogenous PPARα signaling. Highly conserved hydrophobic and charged residues ...

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