High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution

Clinical Chemistry | October 1, 2005| | Copyright

Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described.

Methods: Asymmetric PCR was performed in the presence of unlabeled oligonucleotide probes and a saturating fluorescent DNA dye. High-resolution melting curves for samples in either capillaries (0.3 °C/s) or microtiter format (0.1 °C/s) were generated in the same containers used for amplification. Melting curves of ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Lesotho Unit Trust - Part of a New Dawn for Lesotho.
News Wire article from: Africa News Service ; ...in developing the money markets in Lesotho as well as creating and encouraging...Deputy Governor of the Central Bank of Lesotho, Tabo Foulo, said during the celebrations of the 2nd birthday of the Lesotho Unit Trust on Thursday in Maseru...
Lesotho: the promise of Africa.(PROMOTION)(Country overview)
Magazine article from: Foreign Policy ; ...OMITTED] INVESTMENT IN AFRICA'S GUIDING LIGHT Lesotho is a country paving a new path for development...upcoming branding initiative to make "Made in Lesotho" a global trademark, Lesotho is quickly becoming an African investment destination...
Lesotho: Africa's best kept secret. (An IC Special Report).
Magazine article from: New African ; Pure Majesty If you haven't been to Lesotho yet, you haven't seen natural beauty...deserved. For in some parts of the 75% of Lesotho dominated by the beautiful rugged mountains...the riverine system of Southern Africa. Lesotho is the birthplace of many of the region...
LESOTHO ELECTION: 'NO SENSE IN VOTING IF LIVES REMAIN THE SAME'
News Wire article from: Inter Press Service English News Wire ; ...issued this week show that the ruling Lesotho Congress for Democracy (LCD) has swept...LCD, won 21 of these seats, while the Lesotho Workers Party gained 10. The latter is...just returned from monitoring the poll in Lesotho. He spoke to IPS about the vote, and...
Lesotho-Africa's best kept secret: Pure majesty
Magazine article from: New African ; If you haven't been to Lesotho yet, you haven't seen natural beauty...For, in some parts of the 75% of Lesotho dominated by the beautiful rugged mountains...riverine system of Southern Africa. Lesotho is the birthplace of many of the region...
Lesotho's head of government to visit United States October 7-12. (Justin Meysing Lekhanya)
PR Newswire ; LESOTHO'S HEAD OF GOVERNMENT TO VISIT UNITED...PRNewswire/ -- The Embassy of the Kingdom of Lesotho issued the following: Major-General...the head of government of the Kingdom of Lesotho, will visit the United States Oct. 7...
LESOTHO ELECTION: 'NO SENSE IN VOTING IF LIVES REMAIN THE SAME'.
News Wire article from: Interpress Service ; ...issued this week show that the ruling Lesotho Congress for Democracy (LCD) has swept...LCD, won 21 of these seats, while the Lesotho Workers Party gained 10. The latter is...just returned from monitoring the poll in Lesotho. He spoke to IPS about the vote, and...
Final Lesotho Bank Sale Agreement Signed.
News Wire article from: Africa News Service ; ...November 16, 1999) The Privatisation Unit of the Government of Lesotho and Standard Bank Lesotho limited, on Saturday signed the final agreement effecting the sale of Lesotho Bank. The new company which resumed work in August this year is...
Profile: Lesotho feeling the effects of World Trade Organization's new rules concerning the global textile industry
Transcript from: NPR All Things Considered ; ...Considered (NPR) 01-31-2005 Profile: Lesotho feeling the effects of World Trade Organization...far away as the tiny African nation of Lesotho. As NPR's Jason Beaubien reports...easy to find the front gate of any of Lesotho's garment factories; just look for...
POLITICS-LESOTHO: MOKHEHLE'S DEATH ENDS A TURBULENT ERA
News Wire article from: Inter Press Service English News Wire ; ...controversial former prime minister of Lesotho, Ntsu Mokhehle, on Jan. 6 marked the...had been declared life president of the Lesotho Congress for Democracy (LCD) party. Mokhehle, who headed the government of Lesotho between 1993 and 1998, died at the age...

Find more facts and information related to the article "High-Resolution DNA Melting Analysis for ..."