ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST

In Vitro Cellular & Developmental Biology | May 1, 2005| | Copyright

SUMMARY

A cell line, BPE-1, was derived from a parthenogenetic 8-d in vitro-produced bovine blastocyst that produced a cell outgrowth on STO feeder cells. The BPE-1 cells resembled visceral endoderm previously cultured from blastocysts produced by in vitro fertilization (IVF). Analysis of the BPE-1 cells demonstrated that they produced serum proteins and were negative for interferon-tau production (a marker of trophectoderm). Transmission electron microscopy revealed that the cells were a polarized epithelium connected by complex junctions resembling tight junctions in conjunction with ...

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