Gene Trap Lines Define Domains of Gene Regulation in Arabidopsis Petals and Stamens(w)

From: Plant Cell | Date: September 1, 2005| Author: Arroyo, Juana M; Et al; May, Bruce; Simorowski, Joseph; Nakayama, Naomi | Copyright information

To identify genes involved in Arabidopsis thaliana petal and stamen organogenesis, we used a gene trap approach to examine the patterns of reporter expression at each stage of flower development of 1765 gene trap lines. In 80 lines, the reporter gene showed petal- and/or stamen-specific expression or lack of expression, or expression in distinct patterns within the petals and/or the stamens, including distinct suborgan domains of expression, such as tissue-specific lines marking epidermis and...

Related newspaper, magazine, and journal articles from HighBeam Research

Gene Trap Lines Define Domains of Gene Regulation in Arabidopsis Petals and Stamens(w)
Plant Cell ; To identify genes involved in Arabidopsis thaliana petal and stamen organogenesis, we used a gene trap approach to examine the patterns of reporter expression at each stage of flower development of 1765 gene trap lines. In 80 lines, the reporter gene showed petal- and/or stamen-specific expression
Secretion trap tagging of secreted and membrane-spanning proteins using Arabidopsis gene traps1
Plant Physiology ; Secreted and membrane-spanning proteins play fundamental roles in plant development but pose challenges for genetic identification and characterization. We describe a "secretion trap" screen for gene trap insertions in genes encoding proteins routed through the secretory pathway. The gene trap
Gene and Enhancer Trap Tagging of Vascular-Expressed Genes in Poplar Trees
Plant Physiology ; We report a gene discovery system for poplar trees based on gene and enhancer traps. Gene and enhancer trap vectors carrying the [beta]-glucuronidase (GUS) reporter gene were inserted into the poplar genome via Agrobacterium tumefaciens transformation, where they reveal the expression pattern of
Gene traps: Tools for plant develoopment and genomics
Plant Cell ; INTRODUCTION Classical genetic approaches to gene identification rely on disruption of a gene leading to a recognizable phenotype. This approach continues to be an extremely successful one, yielding mutations that result in overt phenotypes reflecting the function of the corresponding gene. Not all
Trans Genic, Vital Star Conclude Joint Research Agreement for Knockout Mice
JCNN News Summaries - Japan Corporate News Network ; Tokyo, May 30, 2005 - (JCNN) - Trans Genic announced on May 30 that it signed a joint research agreement with Vital Star, a Beijing- based bio venture company born from the Beijing Laboratory Animal Research Center, a research institute jointly set up by the Chinese Government and the Beijing
Functional Analysis of the Tandem-Duplicated P450 Genes SPS/BUS/CYP79F1 and CYP79F2 in Glucosinolate Biosynthesis and Plant Development by Ds Transposition-Generated Double Mutants1
Plant Physiology ; A significant fraction (approximately 17%) of Arabidopsis genes are members of tandemly repeated families and pose a particular challenge for functional studies. We have used the Ac-Ds transposition system to generate single- and double-knockout mutants of two tandemly duplicated cytochrome P450
T-DNA insertional mutagenesis for activation tagging in rice
Plant Physiology ; T-DNA Insertional Mutagenesis for Activation Tagging in Rice1 We have developed a new T-DNA vector, pGA2715, which can be used for promoter trapping and activation tagging of rice (Oryza sativa) genes. The binary vector contains the promoterless 03-glucuronidase (GUS) reporter gene next to the
LucTrap Vectors Are Tools to Generate Luciferase Fusions for the Quantification of Transcript and Protein Abundance in Vivo1
Plant Physiology ; Proper plant growth and development strongly rely on the plant's ability to respond dynamically to signals and cues from the intra- and extracellular environment. Whereas many of these responses require specific changes at the level of gene expression, in recent years it has become increasingly
OsCSLD1, a Cellulose Synthase-Like D1 Gene, Is Required for Root Hair Morphogenesis in Rice1[C][W]
Plant Physiology ; Root hairs are long tubular outgrowths that form on the surface of specialized epidermal cells. They are required for nutrient and water uptake and interact with the soil microflora. Here we show that the Oryza saliva cellulose synthase-like D1 (OsCSLD1) gene is required for root hair development,
The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies
Genetics ; ABSTRACT Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell