RNA Silencing of Single and Multiple Members in a Gene Family of Rice1[w]

From: Plant Physiology | Date: August 1, 2005| Author: Itoh, Rika; Shimamoto, Ko; Miki, Daisuke | Copyright information

RNA silencing with inverted repeat (IR) constructs has been used to suppress gene expression in various organisms. However, the transitive RNA-silencing effect described in plants may preclude the use of RNA silencing for a gene family. Here, we show that, in rice (Oryza saliva), transitive RNA silencing (spreading of double-stranded RNA along the target mRNA) occurred with the green fluorescent protein transgene but not with the endogenous phytoene desaturase gene. We fused IR copies of uniq...

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