Imaging of Photodynamically Generated Singlet Oxygen Luminescence In Vivo¶

Photochemistry and Photobiology | July 1, 2005| | Copyright

ABSTRACT

We describe a novel scanning-laser system for imaging type-II photodynamically generated singlet oxygen (^sup 1^O^sub 2^[^sup 1^Δ^sub g^]) luminescence and demonstrate it in vivo in an intradermal tumor model in mice. We verify the strong oxygen-dependence of the signal and show that the images are near the practical resolution limit.

Abbreviations: ^sup 1^O^sub 2^, singlet oxygen; ALA, δ-aminolevulinic acid; CCD, charge-coupled device; D^sub 2^O, deuterium oxide; Nd:YAG, neodymium; yttrium-lithium-fluoride; NIR, near-infrared; PDT, photodynamic therapy; PMT, ...

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