Atypical Melting Curve Resulting from Genetic Variation in the 3' Untranslated Region at Position 20218 in the Prothrombin Gene Analyzed with the LightCycler Factor II (Prothrombin) G20210A Assay

From: Clinical Chemistry | Date: August 1, 2005| Author: | Copyright information

To the Editor:

Carriers of the G20210A allele have high plasma prothrombin concentrations, which in turn are associated with an almost 3-fold increased risk of venous thrombosis (1). As it is the second most common genetic change associated with inherited thrombophilia, the variant site in the 3' untranslated region is routinely examined in at-risk patients. We use the factor II (prothrombin) G20210A assay (Roche Diagnostics) for genotyping. In this assay, a 165-bp fragment of the prothrombin gene is amplified, and the different allelic variants are distinguished by melting-curve analysis ...

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