Effects of Sucrose on the Internal Dynamics of Azurin Biophysical Journal | June 1, 2005| | Copyright Biophysical Society Jun 2005. Provided by ProQuest LLC. (Hide copyright information) Copyright

ABSTRACT

Sucrose is a natural osmolyte accumulated in cells of organisms as they adapt to environmental stresses. In vitro, sucrose increases protein stability and forces partially unfolded structures to refold. Its effects on the native fold structure and dynamics are not fully established. This study, utilizing Trp phosphorescence spectroscopy, examined the influence of molar concentrations of sucrose on the flexibility of metal-free azurin from Pseudomonas aeruginosa. In addition, by means of specific mutants of the test protein, namely I7S, F110S, and C3A/C26A, that altered its ...

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