Noninvasive Monitoring of Target Gene Expression by Imaging Reporter Gene Expression in Living Animals Using Improved Bicistronic Vectors From: The Journal of Nuclear Medicine | Date: April 1, 2005| Author: Wang, Yanling; Iyer, Meera; Annala, Alexander J; Chappell, Steve; Et al | Copyright Society of Nuclear Medicine Apr 2005. Provided by ProQuest LLC.Copyright information

Indirect, noninvasive imaging of therapeutic gene expression based on levels of reporter gene expression is a powerful tool to devise improved therapeutic strategies in cancer gene therapy. The use of bicistronic vectors carrying internal ribosome entry sites (IRESs) allows the coexpression of multiple gene products from the same promoter but leads to considerable attenuation of the downstream gene. In this study, we describe the use of 10 linked copies of the Gtx (homeodomain protein) IRES (...