CHARACTERIZATION OF CULTURED INSECT CELLS SELECTED BY BACILLUS THURINGIENSIS CRYSTAL TOXIN From: In Vitro Cellular & Developmental Biology | Date: November 1, 2004| Author: Liu, Kaiyu; Zheng, Binglian; Hong, Huazhu; Jiang, Caifu; Et al | Copyright Society for In Vitro Biology Nov/Dec 2004. Provided by ProQuest LLC.Copyright information

SUMMARY

Selection for resistance against Bacillus thuringiensis (Bt) Cry1Ac10 in the Trichoplusia ni (Hübner) cell line BTI-TN-5B1-4 (TnH5) was tested, and the development of resistance in the selected cells was like a S-form curve. Monitoring at the Cry1Ac10 50th challenge, the resistance ratio was 1, 294-fold as many as that of initial cells. But the resistance to Cry1Ac10 declined gradually when the selection was relaxed. The resistance declined rapidly at the low level of resistan...