A SIMPLE WHEAT HAPLOID AND DOUBLED HAPLOID PRODUCTION SYSTEM USING ANTHER CULTURE

From: In Vitro Cellular & Developmental Biology | Date: January 1, 2005| Author: Baenziger, P Stephen; Kim, Kyung-Moon | Copyright information

SUMMARY

Wheat (Triticum aestivum L.) haploids and doubled haploids have been used in breeding programs and genetic studies. Wheat haploids and doubled haploids via anther culture are usually produced by a multiple step culture procedure. We improved a wheat haploid and doubled haploid production system via anther culture in which plants are produced from microspore-derived embryos using one medium and one culture environment. In the improved protocol, tillers of donor plants were pretr...

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