Structural Features of a Hyperthermostable Endo-[beta]-1,3-glucanase in Solution and Adsorbed on "Invisible" Particles Biophysical Journal | January 1, 2005| | Copyright Biophysical Society Jan 2005. Provided by ProQuest LLC. (Hide copyright information) Copyright

ABSTRACT

Conformational characteristics and the adsorption behavior of endo-[beta]-1,3-glucanase from the hyperthermophilic microorganism Pyrococcus furiosus were studied by circular dichroism, steady-state and time-resolved fluorescence spectroscopy, and calorimetry in solution and in the adsorbed state. The adsorption isotherms were determined on two types of surfaces: hydrophobic Teflon and hydrophilic silica particles were specially designed so that they do not interact with light and therefore do not interfere with spectroscopic measurements. We present the most straightforward method to ...

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