High Count Rates with Total Internal Reflection Fluorescence Correlation Spectroscopy

From: Biophysical Journal | Date: January 1, 2005| Author: Hassler, Kai; Anhut, Tiemo; Rigler, Rudolf; GÃ ¶ sch, Michael; Lasser, Theo | Copyright information

ABSTRACT

We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epiillumination configuration.

Fluorescence correlation spectroscopy (FCS) (1) requires a particularly high photon countrate per molecule (cpm) and low background when employed to investigate single molecule dynamics. To fulfill this requirement,...

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