Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157: H7, Vibrio cholerae O1, and Salmonella Typhi

From: Clinical Chemistry | Date: November 1, 2004| Author: Gong, Zhilong; Li, Xing-Fang; Morin, Nicole J | Copyright information

Background: Escherichia coli O157:H7, Vibrio cholcrae O1, and Salmonella Typhi are pathogenic bacteria that can be found in contaminated water supplies throughout the world. No currently available assays can simultaneously detect and identify all three pathogens. Our aim was to develop a rapid and reliable technique for simultaneous detection of these pathogens.

Methods: Four unique genes were chosen as the targets of detection. Forward and reverse primers were designed to specifically...

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