Measurement of Bovine IgG by Indirect Competitive ELISA as a Means of Detecting Milk Adulteration

From: Journal of Dairy Science | Date: March 1, 2004| Author: Coleman, R C; Ireland, H E; Williams, J H H; Hurley, I P | Copyright information

ABSTRACT

The aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows' milk in the milk of goat, sheep, and buffalo. The assay uses a monoclonal antibody produced against bovine IgG. This antibody recognizes a species-specific epitope on the heavy chain of both bovine IgG^sub 1^ and IgG^sub 2^. A peroxidase-conjugated anti-mouse IgG antibod...

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