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Molecular heterogeneity of O-acetylserine sulfhydrylase by two-photon excited fluorescence fluctuation spectroscopy
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ABSTRACT 0-acetylserine sulfhydrylase, a homo-dimeric enzyme from Salmonella typhimurium, covalently binds one pyridoxal 5'-phosphate molecule per subunit as a fluorescent coenzyme. Different tautomers of the Schiff base between the coenzyme and lysine 41 generate structured absorption and fluorescence spectra upon one-photon excitation. We investigated the protein population heterogeneity by fluorescence correlation spectroscopy and lifetime techniques upon two-- photon excitation. We sampled the fluorescence intensity from a small number of molecules (~10) and analyzed the distribution of ...
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The National Church in Local Perspective: The Church of England and the Regions, 1660-1800.(Book Review)
Magazine article from: Church History
; ...contrast, reflected for a time the "patronage, piety, and largess" (95) of a lay network centered on Thomas Pelham Holles, Duke of Newcastle. For the Church in Wales, it was beneficial in the first half of the period to have leaders who were...
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Gillian Glover
Newspaper article from: The Scotsman
; ...quite a man o' pairts in the mid-14th century) Sampson Gideon, an 18th-century gambler, and Sir Thomas Pelham-Holles, Duke of Newcastle. What will be next? Cleopatra's monthly income? The capital transfer tax still owed by Genghis...
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They've dished out enough punishment
Newspaper article from: The Independent - London
; ...we feel nowadays about eating horsemeat. Thomas Pelham- Holles, the Duke of Newcastle and a future Whig Prime Minister, listened...Runners were up and, indeed, running. And the Duke of Newcastle never found out that the blackguards who assaulted...
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