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Single-Tube Method for Nucleic Acid Extraction, Amplification, Purification, and Sequencing
From:
Clinical Chemistry
| Date:
October 1, 2004| Author:
Margraf, Rebecca L; Page, Sam; Erali, Maria; Wittwer, Carl T
| Copyright American Association for Clinical Chemistry Oct 2004. Provided by ProQuest LLC.Copyright information
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Background: The hepatitis C virus (HCV) genotype determines patient prognosis and duration of treatment, but sequencing of the gene is lengthy and labor-intensive. We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping.
Methods: HCV RNA was purified and amplified in tubes coated with a solid-phase matrix that irreversibly bound nucleic acid during the extraction step. After reverse transcriptio...
