Purification and Characterization of Enzymes Exhibiting [beta]-D-Xylosidase Activities in Stem Tissues of Arabidopsis1 From: Plant Physiology | Date: June 1, 2004| Author: Do, Cao Trung; Jouanin, Lise; Lerouge, Patrice; Rihouey, Christophe; Minic, Zoran | Copyright American Society of Plant Physiologists Jun 2004. Provided by ProQuest LLC.Copyright information

This work describes the purification and characterization of enzymes that exhibit [beta]-D-xylosidase activity in stem tissues of Arabidopsis. This is the first detailed investigation that concerns the characterization of catalytic properties and sequence identity of enzymes with [beta]-D-xylosidase activities in a dicotyledonous plant. Three different enzymes, ARAf, XYL4, and XYL1 with apparent molecular masses of 75, 67, and 64 kD, respectively, were purified to homogeneity. ARAf was identi...

Related newspaper, magazine, and journal articles from HighBeam Research

	A Novel Screening Method for Cell Wall Mutants in Aspergillus niger Identifies UDP-Galactopyranose Mutase as an Important Protein in Fungal Cell Wall Biosynthesis Genetics ; ABSTRACT To identify cell wall biosynthetic genes in filamentous fungi and thus potential targets for the discovery of new antifungals, we developed a novel screening method for cell wall mutants. It is based on our earlier observation that the Aspergillus niger agsA gene, which encodes a putative
	The Arabidopsis Root Hair Cell Wall Formation Mutant lrx1 Is Suppressed by Mutations in the RHM1 Gene Encoding a UDP-L-Rhamnose Synthase(W) Plant Cell ; Cell and cell wall growth are mutually dependent processes that must be tightly coordinated and controlled. LRR-extensin1 (LRX1) of Arabidopsis thaliana is a potential regulator of cell wall development, consisting of an N-terminal leucine-rich repeat domain and a C-terminal extensin-like domain
	Rapid structural phenotyping of plant cell wall mutants by enzymatic oligosaccharide fingerprinting Plant Physiology ; Breakthrough Technologies Rapid Structural Phenotyping of Plant Cell Wall Mutants by Enzymatic Oligosaccharide Fingerprinting1 Various biochemical, chemical, and microspectroscopic methods have been developed throughout the years for the screening and identification of mutants with altered cell
	Changes in cell wall polysaccharides of green bean pods during development Plant Physiology ; The changes in cell wall polysaccharides and selected cell wall-- modifying enzymes were studied during the development of green bean (Phaseolus vulgaris L.) pods. An overall increase of cell wall material on a dry-weight basis was observed during pod development. Major changes were detected in the
	Identification of Novel Genes in Arabidopsis Involved in Secondary Cell Wall Formation Using Expression Profiling and Reverse Genetics Plant Cell ; Forward genetic screens have led to the isolation of several genes involved in secondary cell wall formation. A variety of evidence, however, suggests that the list of genes identified is not exhaustive. To address this problem, microarray data have been generated from tissue undergoing secondary
	Impairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease Resistance(W) Plant Cell ; Cellulose Is synthesized by cellulose syntheses (CESAs) contained in plasma membrane-localized complexes. In Arabidopsis thaliana, three types of CESA subunits (CESA4/IRREGULAR XYLEMS [IRX5], CESA7/IRX3, and CESA8/IRX1) are required for secondary cell wall formation. We report that mutations in
	Cell-wall composition of maize internodes of varying maturity. Crop Science ; Lignification of the cell wall during normal plant development is considered the primary factor limiting microbial fermentation of cell-wall polysaccharides in the rumen of cattle (Bos sp.; Jung and Deetz, 1993). Because forage crops represent the major feed resource for cattle production systems,
	UDP-Glucose 4-Epimerase Isoforms UGE2 and UGE4 Cooperate in Providing UDP-Galactose for Cell Wall Biosynthesis and Growth of Arabidopsis thaliana(W)(OA) Plant Cell ; Five Arabidopsis thaliana genes that encode UDP-glucose 4-epimerase (UGE) and represent two ancient plant UGE clades might be involved in the regulation of cell wall carbohydrate biosynthesis. We tested this hypothesis in a genome-wide reverse genetic study. Despite significant contributions of
	MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize 1[W] Plant Physiology ; An extensive search for maize (Zea mays) genes involved in cell wall biosynthesis and assembly has been performed and 735 sequences have been centralized in a database, MAIZEWALL (httpwww.polebio.scsv.ups-tlse.fr/MAIZEWALL). MAIZEWALL contains a bioinformatic analysis for each entry and gene
	Quantitative trait loci and comparative genomics of cereal cell wall composition1 Plant Physiology ; Quantitative trait loci (QTLs) affecting sugar composition of the cell walls of maize (Zea mays) pericarp were mapped as an approach to the identification of genes involved in cereal wall biosynthesis. Mapping was performed using the IBM (B73 x Mo17) recombinant inbred line population. There were

See all results.