How Does Protein Architecture Facilitate the Transduction of ATP Chemical-Bond Energy into Mechanical Work? The Cases of Nitrogenase and ATP Binding-Cassette Proteins From: Biophysical Journal | Date: August 1, 2004| Author: Beratan, David N; Liao, Jie-Lou | Copyright Biophysical Society Aug 2004. Provided by ProQuest LLC.Copyright information

ABSTRACT

Transduction of adenosine triphosphate (ATP) chemical-bond energy into work to drive large-scale conformational changes is common in proteins. Two specific examples of ATP-utilizing proteins are the nitrogenase iron protein and the ATP binding-cassette transporter protein, BtuCD. Nitrogenase catalyzes biological nitrogen fixation whereas BtuCD transports vitamin B12 across membranes. Both proteins drive their reactions with ATP. To interpret how the mechanical force generated ...

Related newspaper, magazine, and journal articles from HighBeam Research

	How Does Protein Architecture Facilitate the Transduction of ATP Chemical-Bond Energy into Mechanical Work? The Cases of Nitrogenase and ATP Binding-Cassette Proteins Biophysical Journal ; ABSTRACT Transduction of adenosine triphosphate (ATP) chemical-bond energy into work to drive large-scale conformational changes is common in proteins. Two specific examples of ATP-utilizing proteins are the nitrogenase iron protein and the ATP binding-cassette transporter protein, BtuCD.
	Nitrogen fixation: The mechanism of the Mo-dependent nitrogenase Critical Reviews in Biochemistry and Molecular Biology ; ABSTRACT: This review focuses on recent developments elucidating the mechanism of the Mo-dependent nitrogenase. This enzyme, responsible for the majority of biological nitrogen fixation, is composed of two component proteins called the MoFe protein and the Fe protein. Recent progress in
	A tale of two transporters The Scientist ; hot PAPERS The first high-resolution structures both please and puzzle the ABC transporter field The ATP binding cassette transporters epitomize nature's ability to re-use a successful protein motif. With diverse membrane-spanning regions, but highly conserved soluble ATP binding domains, the ABC
	The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins with ATPase activity Plant Cell ; Most plant disease resistance (R) genes known today encode proteins with a central nucleotide binding site (NBS) and a C-terminal Leu-rich repeat (LRR) domain. The NBS contains three ATP/GTP binding motifs known as the kinase-1a or P-loop, kinase-2, and kinase-3a motifs. In this article, we show
	Molecular basis for Kir6.2 channel inhibition by adenine nucleotides Biophysical Journal ; ABSTRACT K^sub ATP^ channels are comprised of a pore-forming protein, Kir6.x, and the sulfonylurea receptor, SURx. Interaction of adenine nucleotides with Kir6.2 positively charged amino acids such as K185 and R201 on the C-terminus causes channel closure. Substitution of these amino acids with
	Mutation of a Conserved Glycine in the SH1-SH2 Helix Affects the Load-Dependent Kinetics of Myosin Biophysical Journal ; ABSTRACT The ATP hydrolysis rate and shortening velocity of muscle are load-dependent. At the molecular level, myosin generates force and motion by coupling ATP hydrolysis to lever arm rotation. When a laser trap was used to apply load to single heads of expressed smooth muscle myosin (S1), the ADP
	Distinct Roles for the Saccharomyces cerevisiae Mismatch Repair Proteins in Heteroduplex Rejection, Mismatch Repair and Nonhomologous Tail Removal Genetics ; ... in ATP hydrolysis (KiJAS et al 2003). The msh2-R730Wmutation maps to a region on the Ta^r MutS crystal structure near residues ... adjacent subunit (KiJAS et al. 2003). The msh2-S656P mutation maps to a region on the Taq MutS crystal structure that is ^7 A from ...
	Guanylate Kinase, Induced Fit, and the Allosteric Spring Probe Biophysical Journal ; ABSTRACT Since the introduction of the induced-fit theory by D. E. Koshland Jr., it has been established that conformational motion invariably accompanies the execution of protein function. The catalytic activity of kinases, specifically, is associated with large conformational changes (~1 nm
	Molecule-size motors. (Technology Watch).(Brief Article) Popular Mechanics ; RNA molecules similar to those that cells use to decode DNA can be used to make ultrasmall motors. I think RNA can be made to do mechanical work, says Peixuan Guo, head of a research team at Purdue University in West Lafayette, Ind. ATP binding could power a motor made of six strands of RNA. ATP is
	Simulation of the Coupling between Nucleotide Binding and Transmembrane Domains in the ATP Binding Cassette Transporter BtuCD Biophysical Journal ; ABSTRACT The nucleotide-induced structural rearrangements in ATP binding cassette (ABC) transporters, leading to substrate translocation, are largely unknown. We have modeled nucleotide binding and release in the vitamin B^sub 12^ importer BtuCD using perturbed elastic network calculations and

See all results.