Fluorescence Correlation Spectroscopy Relates Rafts in Model and Native Membranes From: Biophysical Journal | Date: August 1, 2004| Author: Kahya, Nicoletta; Scherfeld, Dag; Schwille, Petra; Bacia, Kirsten | Copyright Biophysical Society Aug 2004. Provided by ProQuest LLC.Copyright information

ABSTRACT

The lipid raft model has evoked a new perspective on membrane biology. Understanding the structure and dynamics of lipid domains could be a key to many crucial membrane-associated processes in cells. However, one shortcoming in the field is the lack of routinely applicable techniques to measure raft association without perturbation by detergents. We show that both in cell and in domain-exhibiting model membranes, fluorescence correlation spectroscopy (FCS) can easily distingui...

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