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Phosphate Binding in the Active Site of Alkaline Phosphatase and the Interactions of 2-Nitrosoacetophenone with Alkaline Phosphatase-Induced Small Structural Changes
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ABSTRACT
To monitor structural changes during the binding of Pi to the active site of mammalian alkaline phosphatase in water medium, reaction-induced infrared spectroscopy was used. The interaction of Pi with alkaline phosphatase was triggered by a photorelease of ATP from the inactive P^sup 3^-[1-(2-nitrophenyl)]ethyl ester of ATP. After photorelease, ATP was sequentially hydrolyzed by alkaline phosphatase giving rise to adenosine and three Pi. Although a phosphodiesterase activity was detected prior the photorelease of ATP, it was possible to monitor the structural effects induced by Pi ...
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Association of alkaline phosphatase phenotypes with arthritides
; ...between 40-70 years of age. Although alkaline phosphatase is found in many tissues, the highest...biliary tract epithelium, and bone. Alkaline phosphatase is a nonspecific phosphomonoesterase...transport takes place, indicating that alkaline phosphatase is involved in fundamental ...
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Multiple unfolding intermediates of human placental alkaline phosphatase in equilibrium urea denaturation
; ABSTRACT Alkaline phosphatase is an enzyme with a typical...stability of the human placental alkaline phosphatase was examined with the chemical...denaturant. INTRODUCTION Alkaline phosphatase (orthophosphoric monoester...
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Blood test alerts patient, doctor to bone problem
; ...249, my triglycerides 199 and my alkaline phosphatase 364. All of these were marked high...right track. I'll stick with the alkaline phosphatase test and your Paget's disease. Although...listed as part of the liver test, the alkaline phosphatase is also a bone test. Yes, Paget...
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NEONATE WITH PERINATAL HYPOPHOSPHATASIA
; ...the tissue non-specific alkaline phosphatase isoenzyme (TNSALP) and by...also depicted. The serum alkaline phosphatase of the baby was very low, 15 IU/L, and a low serum alkaline phosphatase was also found in both his...
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Abnormal liver function tests in asymptomatic patients.
; ...aspartate aminotransferase (AST) or alkaline phosphatase levels. Two reasons cited by the...tests are serum aminotransferases, alkaline phosphatase, bilirubin and albumin determinations...indicator of viral hepatitis.(5) ALKALINE PHOSPHATASE AND GGT Bile formation and flow...
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Hay fever and heredity cause dark circles under eyes
; ...blood-profile tests, I have high alkaline phosphatase. I guess it's nothing to worry about...with someone else's opinion. A. Alkaline phosphatase is an enzyme found in liver, bone...to say that a significant rise of alkaline phosphatase in the blood can be ignored. In...
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Labels Don't Always Reveal Actual Amount of Calcium
; ...Q. For years, I have had a high alkaline phosphatase level, according to blood tests...A. When there's a rise in blood alkaline phosphatase, you look to the liver or to the...no signs of new illness, but the alkaline phosphatase reading remains high. Why? For one...
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Skeletal dysplasias with osteopenia in the newborn: The value of alkaline phosphatase
; Abstract Alkaline phosphatase is a commonly measured enzyme...Skeletal dysplasia, osteopenia, alkaline phosphatase Introduction Osteopenia of...illustrate the value of plasma alkaline phosphatase as an aid in the differential...
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Heat-Labile Alkaline Phosphatase.(NEW technology)
; APex Heat-Labile Alkaline Phosphatase uses a single protocol to dephosphorylate blunt, 5'-overhang...applications require treatment with just 1 m 1 of APex Alkaline Phosphatase, 15 min at 37 C. The enzyme is irreversibly inactivated...
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Shrimp alkaline phosphatase.(usage)(Brief Article)
; ...prevents the re-ligation of linearised plasmid DNA in cloning experiments. Unlike calf intestinal alkaline phosphatase, shrimp alkaline phosphatase is completely and irreversibly inactivated by heat treatment for 15 minutes at 65[degrees...
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