[beta]-1 Integrin-Mediated Adhesion May Be Initiated by Multiple Incomplete Bonds, Thus Accounting for the Functional Importance of Receptor Clustering

From: Biophysical Journal | Date: June 1, 2004| Author: | Copyright information

ABSTRACT

The regulation of cell integrin receptors involves modulation of membrane expression, shift between different affinity states, and topographical redistribution on the cell membrane. Here we attempted to assess quantitatively the functional importance of receptor clustering. We stu died [beta]-1 integrin-mediated attachment of THP-1 cells to fibronectin-coated surfaces under low shear flow. Cells displayed multiple binding events with a half-life of the order of 1 s. The duration of binding events after the first second after arrest was quantitatively accounted for by a model assuming ...

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