High-Frequency Transformation of Undeveloped Plastids in Tobacco Suspension Cells

From: Plant Physiology | Date: May 1, 2004| Author: Broyles, Debra L; Duggan, Lisa L; Et al; Ye, Guang-Ning; Langbecker, Camri L | Copyright information

Although leaf chloroplast transformation technology was developed more than a decade ago, no reports exist of stable transformation of undeveloped plastids or other specialized plastid types, such as proplastids, etioplasts, or amyloplasts. In this work we report development of a dark-grown tobacco suspension cell model system to investigate the transformation potential of undeveloped plastids. Electron microscope analysis confirmed that the suspension cells carry plastids that are significan...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and journal articles from HighBeam Research

High-Frequency Transformation of Undeveloped Plastids in Tobacco Suspension Cells
Plant Physiology ; Although leaf chloroplast transformation technology was developed more than a decade ago, no reports exist of stable transformation of undeveloped plastids or other specialized plastid types, such as proplastids, etioplasts, or amyloplasts. In this work we report development of a dark-grown tobacco
Chloroplast Vector Systems for Biotechnology Applications1
Plant Physiology ; ... resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123: 281-292 Hibberd JM, Linley PJ, Khan MS, Gray JC (1998) Transient expression of green ...
A Guide to Choosing Vectors for Transformation of the Plastid Genome of Higher Plants1[C][W][OA]
Plant Physiology ; Plastid transformation, originally developed in tobacco (Nicotiana tabacum), has recently been extended to a number of crop species enabling in vivo probing of plastid function and biotechnological applications. In this article we report new plastid vectors that enable insertion of transgenes in
Chloroplast Genetic Engineering: Recent Advances and Future Perspectives
Critical Reviews in Plant Sciences ; ... and humans. It was found that cell extracts resulted in 96 percent inhibition of growth of P. aeruginosa, highly encouraging news for AIDS and cystic fibrosis patients who are highly susceptible to the pathogen. Of course, further studies are needed to determine ...
Biochemical and Molecular Characterization of AtPAP26, a Vacuolar Purple Acid Phosphatase Up-Regulated in Phosphate-Deprived Arabidopsis Suspension Cells and Seedlings1
Plant Physiology ; A vacuolar acid phosphatase (APase) that accumulates during phosphate (Pi) starvation of Arabidopsis (Arabidopsis thaliana) suspension cells was purified to homogeneity. The final preparation is a purple APase (PAP), as it exhibited a pink color in solution (A^sub max^ = 520 nm). It exists as a
Persistence of unselected transgenic DNA during a plastid transformation and segregation approach to herbidicide resistance
Plant Physiology ; The use of a nonlethal selection scheme, most often using the aadA gene that confers resistance to spectinomycin and streptomycin, has been considered critical for recovery of plastid transformation events. In this study, the plastid-lethal markers, glyphosate or phosphinothricin herbicides, were
Development of Novel Large Elongated Fiber-Structure in Plant Protoplast Cultures
In Vitro Cellular & Developmental Biology ; P-2121 Novel large elongated fiber-structures were repeatedly found in liquid plant protoplast cultures, e.g., leaves of Betula platyphylla, and embryogenic suspension cells of Larix leptolepis, suspension cells of tobacco BY-2 and Vinca rosea. Micro-staining method was developed using a
Arabidopsis Peroxin 16 Coexists at Steady State in Peroxisomes and Endoplasmic Reticulum1
Plant Physiology ; Homologs of peroxin 16 genes (PEX16) have been identified only in Yarrowia lipolytica, humans (Homo sapiens), and Arabidopsis (Arabidopsis thaliana). The Arabidopsis gene (AtPEX16), previously reported as the SSE1 gene, codes for a predicted 42-kD membrane peroxin protein (AtPexlop). Lin et al. (Y.
Induction of Abscisic Acid-Regulated Gene Expression by Diacylglycerol Pyrophosphate Involves Ca^sup 2+^ and Anion Currents in Arabidopsis Suspension Cells1[W]
Plant Physiology ; Diacylglycerol pyrophosphate (DGPP) was recently shown to be a possible intermediate in abscisic acid (ABA) signaling. In this study, reverse transcription-PCR of ABA up-regulated genes was used to evaluate the ability of DGPP to trigger gene expression in Arabidopsis (Arabidopsis thaliana)
Calgene Receives Biotech Patent For Manipulating Planet Cells
Chemical Market Reporter ; CALGENE INC. has received its third bioengineering patent for manipulating palnt cells by adding foreign genes to their plastids. The technology is an outgrowth of Calgene's research into modifying the oil composition in plants. The company expects the technology to be used in pharmaceuticals,