Evaluation of 15 Polymerases and Phosphorothioate Primer Modification for Detection of UV-induced C:G to T:A Mutations by Allele-specific PCR¶ From: Photochemistry and Photobiology | Date: May 1, 2004| Author: Tafoya, Gregory B; Gale, James M | Copyright American Society of Photobiology May 2004. Provided by ProQuest LLC.Copyright information

ABSTRACT

Allele-specific polymerase chain reaction is based on polymerase extension from primers that contain a 3' end base that is complementary to a specific mutation and inhibition of extension with wild-type DNA due to a 3' end mismatch. Taq polymerase is commonly used for this assay, but because of the high rate of nucleotide extension from primer 3' base mismatches documented for Taq polymerase, high sensitivity is difficult to achieve. To determine whether other polymerases migh...

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